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Seven different monoclonal antibodies specific for tyosine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity.
Recognition is therefore dependent on 2 criteria:
1) phosphorylation
2) the surrounding amino acid motif.
If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site.
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RUO – Research Use Only |
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