Phosphothreonine Detection Set

Three different monoclonal antibodies specific for threonine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity.

Recognition is therefore dependent on 2 criteria:
1) phosphorylation
2) the surrounding amino acid motif.

If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site.