AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Shipping: Available products typically ship within 24/48h, via priority shipping.
Do you need support? Contact Customer Service or Technical Support.
Online Account
Access or Create Your Account
This antibody is covered by our Worry-Free Guarantee.
Product Details
| Application |
ELISA, IP, WB |
|---|---|
| Clone |
16B4 |
| Formulation |
Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
| Host |
Mouse |
| Immunogen |
Phosphoserine containing peptides conjugated to KLH. |
| Isotype |
IgM |
| Positive Control |
Included. (Prod. No. 840-012-1) |
| Purity Detail |
Thiophilic adsorption and size exclusion chromatography purified. |
| Recommendation Dilutions/Conditions |
ELISA (use at 0.05µg/ml)Western Blot (1µg/ml HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution! Replace by BSA!)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Reconstitution |
Reconstitute with 1ml water (15 minutes at room temperature). |
| Species Reactivity |
Species independent |
| Specificity |
Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, strongly interacting with pSK and pSP motifs, thus specifically recognizing substrates of MAP/SAP kinases and CDC kinases. Please note that phosphoserine detection by MAbs always depends on the surrounding aa sequence. |
| Technical Info / Product Notes |
Replacement for ADI-905-805 |
| Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
| Use/Stability |
Stable at -80°C up to 1 year, at 4°C up to 1 week. |
|---|---|
| Handling |
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
| Long Term Storage |
-20°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Regulation of FcRγ function by site-specific serine phosphorylation: Shah, S., Gibson, A. W., et al.; J. Leukoc. Biol. 101, 421 (2017), Abstract
- PKA and CDK5 can phosphorylate specific serines on the intracellular domain of podoplanin (PDPN) to inhibit cell motility.: Liu, T., Li, H., et al.; Exp. Cell Res. 335, 115 (2015), Application(s): WB, Abstract
- Amyloid beta deregulates astroglial mGluR5-mediated calcium signaling via calcineurin and Nf-kB.: Lim, D., Iyer, A., et al.; Glia 61, 1134 (2013), Application(s): WB, Abstract
- Two waves of the nuclear phospholipase C activity in serum-stimulated HL-60 cells during G(1) phase of the cell cycle: Lukinovic-Skudar, V., Matkovic, K., et al.; Biochim. Biophys. Acta 1771, 514 (2007), Abstract
- Protein phosphatase 2A regulates apoptosis in neutrophils by dephosphorylating both p38 MAPK and its substrate caspase 3: M. Alvarado-Kristensson and T. Andersson; J. Biol. Chem. 280, 6238 (2005), Abstract — Full Text
- p38-MAPK signals survival by phosphorylation of caspase-8 and caspase-3 in human neutrophils: M. Alvarado-Kristensson, et al.; J. Exp. Med. 199, 449 (2004), Abstract — Full Text
- Phosphorylation of Bax Ser184 by Akt regulates its activity and apoptosis in neutrophils: S.J. Gardai, et al.; J. Biol. Chem. 279, 21085 (2004), Abstract — Full Text
- Towards a phosphoproteome map of Corynebacterium glutamicum: A.K. Bendt, et al.; Proteomics 3, 1637 (2003), Abstract
- Kennedy’s disease. Phosphorylation of the polyglutamine-expanded form of androgen receptor regulates its cleavage by caspase-3 and enhances cell death: M.A. LaFevre-Bernt and L.M. Ellerby; J. Biol. Chem. 278, 34918 (2003), Abstract — Full Text
- Glyceraldehyde-3-phosphate dehydrogenase is phosphorylated by protein kinase Ciota /lambda and plays a role in microtubule dynamics in the early secretory pathway: E.J. Tisdale; J. Biol. Chem. 277, 3334 (2002), Abstract — Full Text
- Human phosphatidylinositol 4-kinase isoform PI4K92. Expression of the recombinant enzyme and determination of multiple phosphorylation sites: S. Suer, et al.; Eur. J. Biochem. 268, 2099 (2001), Abstract — Full Text
- MID1, mutated in Opitz syndrome, encodes an ubiquitin ligase that targets phosphatase 2A for degradation: A. Trockenbacher, et al.; Nat. Genet. 29, 287 (2001), Abstract — Full Text
- The ternary complex factor Net contains two distinct elements that mediate different responses to MAP kinase signalling cascades: C. Ducret, et al.; Oncogene 19, 5063 (2000), Abstract
- Insulin selectively stimulates nuclear phosphoinositide-specific phospholipase C (PI-PLC) beta1 activity through a mitogen-activated protein (MAP) kinase-dependent serine phosphorylation: A.M. Martelli, et al.; FEBS Lett. 486, 230 (2000), Abstract
Related Products
Datasheet, Manuals, SDS & CofA
Manuals And Inserts
Certificate of Analysis
Please enter the lot number as featured on the product label
SDS
Enzo Life Science provides GHS Compliant SDS
If your language is not available please fill out the SDS request form