Phosphofructokinase Activity Assay

Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role in glycolysis. PFK catalyzes the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate. It is allosterically regulated by several chemicals including ATP, ADP and citrate. When energy levels are low the ADP to ATP ratio is high which turns on PFK, accelerating glycolysis. Conversely when energy levels are high ATP and citrate work to turn off PFK. Dysregulation of PFK leads to metabolic disorders. PFK has been targeted in cancer therapy since cancer cells display enhanced glycolytic activity. AkrivisBio’s Phosphofructokinase Activity Assay is a simple sensitive means of quantifying PFK activity in a variety of biological samples with a sensitivity in the microunit range.