The PGF2α ELISA kit is a colorimetric competitive enzyme immunoassay kit
- Broad dynamic range suitable for many sample types
- Stable liquid color-coded reagents reduce errors
- Convenient storage conditions
- Results from up to 36 samples in duplicate in less than 3 hours
- Reproducible results day-after-day and lot-after-lot
The PGF2α EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Broad dynamic range is suitable for a wide variety of sample types. Ready-to-use liquid color-coded reagents are safe, non-radioactive, and reduce laboratory errors.
Prostaglandin F2α (PGF2α) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF2α is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2α have also been associated with preeclampsia.
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Product Details
Alternative Name |
Prostaglandin F2α |
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Application |
Colorimetric detection, ELISA |
Application Notes |
For the quantitative determination of PGF2α in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample types include cell lysate and whole blood. |
Assay Time |
<3 hours |
Compatibility |
This product is compatible with the Absorbance 96 Plate Reader. |
Contents |
DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
Sensitivity |
6.71 pg/ml (range 3.05 – 50,000 pg/ml) |
Species Reactivity |
Species independent |
Wavelength |
405 nm |
Handling & Storage
Long Term Storage |
+4°C |
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Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- The Autocrine Impact of Nerve Growth Factor on Sheep Uterine Epithelial Cells: Guelfi, G., Pasquariello, R., et al.; Cells 14, (2025), Abstract
- Intrauterine devices influence prostaglandin secretion by equine uterus: in vitro and in vivo studies: Piotrowska-Tomala, K. K., Jonczyk, A. W., et al.; BMC Vet. Res. 20, 46 (2024), Abstract
- Prostaglandin F2α Affects the Cycle of Clock Gene Expression and Mouse Behavior: Tsurudome, Y., Yoshida, Y., et al.; Int. J. Mol. Sci. 25, (2024), Abstract
- The suppression of MAPK/NOX/MMP signaling prompts renoprotection conferred by prenatal naproxen in weaning preeclamptic rats: S.A. Abdelhady, et al.; Sci. Rep. 13, 17498 (2023), Abstract
- The Effects of Prostaglandin E2 Treatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: An in vivo Study: Piotrowska-Tomala, K. K., Jonczyk, A. W., et al.; Front. Vet. Sci. 8, 753796 (2022), Abstract
- Gestational NSAIDs distinctly reprogram cardiac injury in preeclamptic rats: Roles of cyclooxygenase, apoptotic and autophagic trails: M.A. Ali, et al.; Life Sci. 310, 121130 (2022), Abstract
- Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol. 12, 774086 (2022), Application(s): ELISA kit using hemolymph, Abstract — Full Text
- Piezo1 activation induces fibronectin reduction and PGF2α secretion via arachidonic acid cascade: W. Morozumi, et al.; Exp. Eye Res. 215, 108917 (2022), Abstract
- Involvement of bradykinin and bradykinin B1 receptor in patients with endometriosis: Meng, X., Li, Y., et al.; Exp. Ther. Med. 22, 1240 (2021), Abstract
- Inflammatory Markers in Uterine Lavage Fluids of Pregnant, Non-Pregnant, and Intrauterine Device Implanted Mares on Days 10 and 15 Post Ovulation: Rivera Del Alamo, M. M., Reilas, T., et al.; Animals (Basel) 11, (2021), Abstract
- The In Vitro Inhibitory Effect of Sivelestat on Elastase Induced Collagen and Metallopeptidase Expression in Equine Endometrium: Amaral, A., Fernandes, C., et al.; Animals (Basel) 10, (2020), Abstract
- Mechanisms of thrombin-Induced myometrial contractions: Potential targets of progesterone: Nishimura, F., Mogami, H., et al.; PLoS One 15, e0231944 (2020), Abstract
- Lysophosphatidic acid as a regulator of endometrial connective tissue growth factor and prostaglandin secretion during estrous cycle and endometrosis in the mare: Szóstek-Mioduchowska, A. Z., Leciejewska, N., et al.; BMC Vet. Res. 16, 343 (2020), Abstract
- Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation: Robeldo, T., Canzi, E. F., et al.; Sci. Rep. 10, 7063 (2020), Abstract
- The Inhibition of Cathepsin G on Endometrial Explants With Endometrosis in the Mare: Amaral, A., Fernandes, C., et al.; Front. Vet. Sci. 7, 582211 (2020), Abstract
- The role of vaspin in porcine corpus luteum: Kurowska, P., Mlyczyńska, E., et al.; J. Endocrinol. 247, 283 (2020), Abstract
- Effect of proinflammatory cytokines on endometrial collagen and metallopeptidase expression during the course of equine endometrosis: A.Z. Szóstek-Mioduchowska, et al.; Cytokine 123, 154767 (2019), Application(s): Horse endometrial tissue, Abstract
- The role of embryo contact and focal adhesions during maternal recognition of pregnancy: K.M. Klohonatz, et al.; PLoS One 14, e0213322 (2019), Abstract — Full Text
- The influence of experimentally induced endometritis on the PPAR expression profile in the bovine endometrium: B.M. Socha, et al.; Theriogenology 122, 74 (2018), Application(s): ELISA using culture supernatants, Abstract
- Elastase inhibition affects collagen transcription and prostaglandin secretion in mare endometrium during the estrous cycle: A. Amaral, et al.; Reprod. Domest. Anim. 53, 66 (2018), Abstract
- Oxidative Stress is a Convincing Contributor to Idiopathic Chronic Fatigue: J.S. Lee, et al.; Sci. Rep. 8, 12890 (2018), Abstract — Full Text
- Colon cancer-derived myofibroblasts increase endothelial cell migration by glucocorticoid-sensitive secretion of a pro-migratory factor: Z. Drebert, et al.; Vascul. Pharmacol. 89, 19 (2017), Application(s): Determine the concentrations of prostanoids in conditioned medium from myofibroblasts and HUVECs, and in HUVEC lysates, Abstract
- Electrochemical immunosensor for the determination of 8-isoprostane aging biomarker using carbon nanohorns-modified disposable electrodes: E.E Sánchez-Tirado, et al.; J. Electroanal. Chem. 793, 197 (2017)
- Circulating retinoic acid levels and the development of metabolic syndrome: Y. Liu, et al.; J. Clin. Endocrinol. Metab. 101, 1686 (2016), Application(s): Measured plasma 8-iso-Prostaglandin F2, Abstract — Full Text
- Regulation of cyclooxygenase-2 expression in rat oviductal epithelial cells: Evidence for involvement of GPR30/Src kinase-mediated EGFR signaling: P. Popli, et al.; J. Steroid Biochem. Mol. Biol. 154, 130 (2015), Application(s): ELISA using rat cell lysate, Abstract
- Female preference for males depends on reproductive physiology in the African cichlid fish Astatotilapia burtoni: M.R. Kidd, et al.; Gen. Comp. Endocrinol. 180, 56 (2013), Application(s): ELISA using aquarium water, Abstract
- Measuring multiple hormones from a single water sample using enzyme immunoassays: C. Kidd, et al.; Gen. Comp. Endocrinol. 165, 277 (2010), Application(s): EIA using fish plasma and water samples, Abstract
- Discovery of ecopladib, an indole inhibitor of cytosolic phospholipase A2 alpha: K.L. Lee, et al.; J. Med. Chem. 50, 1380 (2007), Application(s): EIA using human whole blood, Abstract
- Cloning, Expression, and Functional Characterization of Human Cyclooxygenase-1 Splicing Variants: Evidence for Intron 1 Retention: N. Qin, et al.; J. Pharmacol. Exp. Ther. 315, 1298 (2005), Application(s): EIA using human cell lysates, Abstract
- 17{beta}-Estradiol decreases vascular tone in cerebral arteries by shifting COX-dependent vasoconstriction to vasodilation: J.A. Ospina, et al.; Am. J. Physiol. Heart Circ. Physiol. 285, H241 (2003), Application(s): EIA using rat cell lysates, Abstract
- Skeletal Muscle PGF2{alpha}and PGE2 in Response to Eccentric Resistance Exercise: Influence of Ibuprofen and Acetaminophen: T.A. Trappe, et al.; J. Clin. Endocrinol. Metab. 86, 5067 (2001), Application(s): EIA using human cell lysates, Abstract
- Tumor Necrosis Factor-alpha Induces Cyclooxygenase-2 Expression and Prostaglandin Release in Brain Microvessel Endothelial Cells: K.S. Mark, et al.; J. Pharmacol. Exp. Ther. 297, 1051 (2001), Application(s): EIA using bovine cell lysates, Abstract
- Phase-dependent influence of nonsteroidogenic cells on steroidogenesis and prostaglandin production by the human corpus luteum: B.E. Friden, et al.; Fertil. Steril. 73, 359 (2000), Application(s): EIA using human culture supernatants, Abstract
- Prostaglandin-Independent Anovulatory Mechanism of Indomethacin Action: Inhibition of Tumor Necrosis Factor {alpha}-Induced Sheep Ovarian Cell Apoptosis: W.J. Murdoch, et al.; Biol. Reprod. 61, 1655 (1999), Application(s): EIA using sheep cell lysates, Abstract
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PGF2α ELISA kit
ADI-901-069
The PGF2α ELISA kit is a colorimetric competitive enzyme immunoassay kit

Application | Colorimetric detection, ELISA |
---|---|
Assay Time | <3 hours |
Sensitivity | 6.71 pg/ml (range 3.05 – 50,000 pg/ml) |
Datasheet, Manuals, SDS & CofA
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