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PGF_2α ELISA kit

ADI-901-069

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The PGF_2α ELISA kit is a colorimetric competitive enzyme immunoassay kit

Broad dynamic range suitable for many sample types
Stable liquid color-coded reagents reduce errors
Convenient storage conditions
Results from up to 36 samples in duplicate in less than 3 hours
Reproducible results day-after-day and lot-after-lot
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The PGF_2α EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Broad dynamic range is suitable for a wide variety of sample types. Ready-to-use liquid color-coded reagents are safe, non-radioactive, and reduce laboratory errors.

Prostaglandin F_2α (PGF_2α) is formed in a variety of cells from PGH₂, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF_2α is often viewed as an antagonist to PGE₂ due to their opposing effects on various tissues. PGF_2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF_2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF_2α have also been associated with preeclampsia.

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Product Details

Alternative Name	Prostaglandin F2α
Application	Colorimetric detection, ELISA
Application Notes	For the quantitative determination of PGF_2α in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample types include cell lysate and whole blood.
Assay Time	<3 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	DxS Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Sensitivity	6.71 pg/ml (range 3.05 – 50,000 pg/ml)
Species Reactivity	Species independent
Wavelength	405 nm

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

The Autocrine Impact of Nerve Growth Factor on Sheep Uterine Epithelial Cells: Guelfi, G., Pasquariello, R., et al.; Cells 14, (2025), Abstract
Intrauterine devices influence prostaglandin secretion by equine uterus: in vitro and in vivo studies: Piotrowska-Tomala, K. K., Jonczyk, A. W., et al.; BMC Vet. Res. 20, 46 (2024), Abstract
Prostaglandin F2α Affects the Cycle of Clock Gene Expression and Mouse Behavior: Tsurudome, Y., Yoshida, Y., et al.; Int. J. Mol. Sci. 25, (2024), Abstract
The suppression of MAPK/NOX/MMP signaling prompts renoprotection conferred by prenatal naproxen in weaning preeclamptic rats: S.A. Abdelhady, et al.; Sci. Rep. 13, 17498 (2023), Abstract
The Effects of Prostaglandin E2 Treatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: An in vivo Study: Piotrowska-Tomala, K. K., Jonczyk, A. W., et al.; Front. Vet. Sci. 8, 753796 (2022), Abstract
Gestational NSAIDs distinctly reprogram cardiac injury in preeclamptic rats: Roles of cyclooxygenase, apoptotic and autophagic trails: M.A. Ali, et al.; Life Sci. 310, 121130 (2022), Abstract
Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol. 12, 774086 (2022), Application(s): ELISA kit using hemolymph, Abstract — Full Text
Piezo1 activation induces fibronectin reduction and PGF2α secretion via arachidonic acid cascade: W. Morozumi, et al.; Exp. Eye Res. 215, 108917 (2022), Abstract
Involvement of bradykinin and bradykinin B1 receptor in patients with endometriosis: Meng, X., Li, Y., et al.; Exp. Ther. Med. 22, 1240 (2021), Abstract
Inflammatory Markers in Uterine Lavage Fluids of Pregnant, Non-Pregnant, and Intrauterine Device Implanted Mares on Days 10 and 15 Post Ovulation: Rivera Del Alamo, M. M., Reilas, T., et al.; Animals (Basel) 11, (2021), Abstract

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Sensitivity	6.71 pg/ml (range 3.05 – 50,000 pg/ml)
Species Reactivity	Species independent

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PGF_2α ELISA kit

ADI-901-069

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

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