PGE₂ FPIA kit

• Higher throughput – homogeneous mix-and-read assay
• Flexible – signal generated can be read after 30 minutes or several hours
• Robust – Z’ Factor >0.8

The PGE₂ FPIA kit is a homogeneous mix-and-read fluorescence polarization enzyme immunoassay kit with. Results are obtained within just 30 minutes and the generated signal is stable for up to 20 hours. The PGE₂ FPIA kit is ideal for high throughput screening (HTS) applications. A convenient homogeneous mix-and-read assay that is amendable to miniaturization and automation, this kit has a robust Z factor >0.8.

Prostaglandin E₂ (PGE₂) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH₂), which is further metabolized by PGE synthases into its major product, PGE₂. PGE₂ mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE₂ has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE₂ production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages.