PGE2 CLIA kit

• Validated in numerous sample matrices
• Results in just 3 hours
• Sensitive to 6.03 pg/ml
• Results from up to 36 samples in duplicate per plate

The PGE₂ CLIA kit is a sensitive chemiluminescent competitive enzyme immunoassay kit for the quantitative determination of PGE₂ in biological fluids from any species.

Prostaglandin E₂ (PGE₂) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH₂), which is further metabolized by PGE synthases into its major product, PGE₂. PGE₂ mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE₂ has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE₂ production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages.