pE-SUMOpro3 Amp Kit

The SUMOpro3 Protein and Peptide Expression Kit maximizes the yield of soluble, untagged, functional proteins with desired N-termini.  While other prokaryotic expression systems use engineered protease cleavage sites that produce amino acid extensions and often result in non-specific cleavage within the protein of interest, LifeSensors’ highly active and specific SUMO Protease 2 precisely recognizes the tertiary structure of the SUMO (hSUMO3) tag, generating the desired N-terminus and never cleaving within your protein of interest. By linking a His6 epitope to both the SUMO-3 fusion tag and SUMO Protease 2, the SUMOpro3 System allows affordable, efficient and rapid purification of untagged protein using immobilized metal affinity chromatography.