PDQ Protease Assay™

The PDQ Protease Assay is a colorimetric assay used to detect protease activity. The proprietary substrate is susceptible to a wide range of proteases, e.g., collagenase, proteinase K, papain, pepsin, bromelin, ficin, trypsin and chymotrypsin. This substrate is a cross-linked matrix containing protein and a dye-protein conjugate. The matrix is supplied in ready-to-use vials. Test samples containing putative protease activity are aliquoted into the vials which are then incubated at an appropriate temperature. Protease activity is detected spectrophotometrically with increasing optical density proportional to increasing enzyme activity.