PCR & Gel Clean-up Kit

Key Features  
     High performance: Recovers up to 95% of DNA fragments down to 50 bp with binding capacity up to 25 µg 
     Advanced: Binding buffer with pH indicator ensuring optimal binding conditions (pH < 7.0) 
     Concentrated recovery: elution volumes as small 15 µl 

PCR & Gel clean-up columns use silica membrane technology in a mini spin column format to deliver easy and efficient DNA purification (removal of unused, single stranded primers) from PCR products, agarose gels, and labeling reactions.

Sample material: <400 µl PCR reaction mixture, <400mg agarose gel 
Fragment size: 50bp – ~20kbp 
Recovery: 70 – 95% 
Elution volume: 15 – 50 µl 
Preparation time: 10 min for 6 preps 
Binding capacity: 25 µg 
Handling: Centrifugation, vacuum 
Typical purity (A260/A280): 1.8 – 1.9

The PCR & Gel Clean-up Kits can be used downstream of DNA labeling with Enzo’s CYTAG^® CGH labeling Kit (Prod. No. ENZ-42671) and are included with the CYTAG^® TotalCGH labeling kit (Prod. No. ENZ-42674) to clean up excess nucleotides and enzymes.

A rapid and simple mini kit for purifying DNA from PCR reactions, agarose and polyacrylamide gels, and labeling reactions. Our silica membrane PCR & Clean-up mini spin columns reliably remove primers, enzymes, mineral oil, PCR additives (e.g., salts, betaine, DMSO), detergents (e.g., Tween 20, Triton X-100), dyes, SDS, unbound labels, and free dNTPs, delivering high quality and pure DNA for use in CGH arrays, PCR, ligation, sequencing, etc.