PCR decontamination kit

• Removes DNA contamination from PCR mastermixes
• Improves target detection by reducing background
• Does not effect PCR sensitivity
• Optimized for PCR and probe based qPCR mixes

The PCR decontamination kit uses a dsDNase to remove contaminating DNA from mastermixes. The double-strand specific property allows decontamination in the presence of primers and probe.The dsDNase is irreversibly inactivated by heating to 60°C in the presence of DTT, ensuring that DNA template added to the mastermix after inactivation will not be digested. The mastermix can be used immediately after decontamination to run PCR reactions.

AMPIGENE^® Taq polymerases have been treated to remove host DNA and additional clean-up is not necessary. The PCR decontamination kit is recommended for decontamination of primers or competitor’s PCR mixes.

Polymerases used in PCR are frequently contaminated with E. coli DNA. Contaminating DNA may cause reduced sensitivity and false positives when small amounts of bacterial DNA are targeted. Other sources of contamination might be dNTPs, buffer components and primers / probes, as well as DNA introduced during handling. The PCR decontamination kit is an easy method to eliminate DNA contamination.