AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Shipping: Available products typically ship within 24/48h, via priority shipping.
Do you need support? Contact Customer Service or Technical Support.
Online Account
Access or Create Your Account
This antibody is covered by our Worry-Free Guarantee.
Western blot analysis of whole cell extracts from (1) human HL60 leukemia cells (Prod. No. BML-SW101] and (2) HL60 cells induced to undergo apoptosis using etoposide (Prod. No. BML-SW102). The membrane was probed with C-2-10 mouse monoclonal antibody (Prod. No. BML-SA250) at 1:1000 dilution, secondary antibody was GAMAP (1:2000) and developed with BCIP/NBT. Arrows correspond to 116kDa and 85kDa.
BPLF1 promotes the selective degradation of nuclear cullins by the proteasome.A. Cullins are selectively degraded during EBV replication. Western blots of cell lysates from induced Akata-Bx1 were probed with the indicated antibodies. One representative experiment out of three is shown. B. The cullins are degraded by the proteasome. Ten µM of the proteasome inhibition MG132 were added to one aliquot of Akata-Bx1 cells 48 h after induction and the cells were culture overnight before western blot analysis with the indicated antibodies. One representative experiment out of three is shown. C. Representative western blots illustrating the changes in expression levels of cytoplasmic and nuclear cullins. Subcellular fractionation was performed at the indicated time after induction and the efficiency of fractionation was confirmed by probing western blots with antibodies to PARP, histone H1 and β-actin. One representative experiment out of three is shown. D. Quantification of the fold change relative to the levels of expression at time 0. The mean ± SE of three experiments are shown. Significant differences between fold changes in the cytoplasmic and nuclear fractions are indicated * = p<0.01, ** = p<0.001.
Image collected and cropped by CiteAb under a CC-BY license from the following publication: Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells. PLoS Pathog (2013)
| Regulatory Status |
RUO – Research Use Only |
|---|
Related Products
| Alternative Name | Poly(ADP-ribose) polymerase-1 |
|---|---|
| Application | ELISA, ICC, WB |
| Host | Mouse |
| Isotype | IgG1 |
| Species Reactivity | Bovine, Hamster, Human, Monkey, Mouse, Rat |
| Alternative Name | Poly(ADP-ribose) polymerase, PARP-1 |
|---|---|
| Application | ELISA, WB |
| Host | Rabbit |
| Species Reactivity | Bovine, Human, Mouse, Rat |