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PARP-1 (human), (recombinant) (high purity) (His-tag)

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Alternative Name	Poly(ADP-ribose) polymerase 1
Formulation	Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
Purity	≥98% (SDS-PAGE)
Source	Produced in Sf21 insect cells. Human full-length PARP-1 is fused to a Myc-tag and a His-tag. Produced in a baculovirus expression system.
Specific Activity	≥1’200U/mg protein.
UniProt ID	P09874

Handling	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Identification of poly (ADP-ribose) polymerase-1 (PARP-1) as a novel Kruppel-like factor 8-interacting and -regulating protein: H. Lu, et al.; J. Biol. Chem. 286, 20335 (2011), Abstract — Full Text
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription: P.O. Hassa, et al.; J. Biol. Chem. 280, 40450 (2005), Abstract — Full Text
Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1: P.O. Hassa, et al.; J. Biol. Chem. 278, 45145 (2003), Abstract — Full Text

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