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Product Details
| Alternative Name |
Poly(ADP-ribose) glycohydrolase |
|---|---|
| Formulation |
Liquid. In 50mM K-PO4 buffer, pH 7.5, containing 50mM potassium chloride, 0.1% Triton-x 100, 10% glycerol and 1mM DTT, 10mM β-Mercaptoethanol. |
| Purity Detail |
Purified by substrate chromatography. The enzyme is composed of two major bands at 74kDa and 59kDa. |
| Source |
Isolated from calf thymus. |
| Specific Activity |
~20’000U/mg protein. 1U is defined as the amount of enzyme that hydrolyzes 1nmol of poly(ADP-ribose) per min. at 37°C. |
| UniProt ID |
O02776 |
Handling & Storage
| Use/Stability |
As indicated on product label or CoA when stored as recommended. For further dilution use the following enzyme dilution buffer: 50mM KPO4, pH 7.5, 50mM KCl, 10 mM β-mercaptoethanol, 10% glycerol, 1mM DTT, 0.1% Triton. |
|---|---|
| Handling |
Avoid freeze/thaw cycles. |
| Long Term Storage |
-80°C |
| Shipping |
Dry Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Reading ADP-ribosylation signaling using chemical biology and interaction proteomics: K.W, Kliza, et al.; Mol. Cell 81, 4552 (2021), Abstract
- Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner: X. Yue, et al.; Commun. Biol. 4, 1370 (2021), Abstract
- Poly(ADP-ribosylation) is present in murine sciatic nerve fibers and is altered in a Charcot-Marie-Tooth-1E neurodegenerative model: L.I. Hughes, et al.; PeerJ 5, e3318 (2017), Abstract — Full Text
- Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE): D.S. Pitcher, et al.; Biochim. Biophys. Acta 1844, 2222 (2014), Abstract
- A family of macrodomain proteins reverses cellular mono-ADP-ribosylation: G. Jankevicius, et al.; Nat. Struct. Mol. Biol. 20, 508 (2013), Abstract — Full Text
- Methods for biochemical study of poly(ADP-ribose) metabolism in vitro and in vivo: G.M. Shah et al.; Anal. Biochem. 227, 1 (1995), Abstract
- Mode of action of poly(ADP-ribose) glycohydrolase: G. Brochu, et al.; Biochim. Biophys. Acta 1219, 342 (1994), Abstract
- Purification of poly(ADP-ribose) glycohydrolase and detection of its isoforms by a zymogram following one- or two-dimensional electrophoresis: G. Brochu et al.; Anal. Biochem 218, 265 (1994), Abstract
- Poly(ADP-ribosyl)ation of chromatin in an in vitro poly(ADP-ribose)-turnover system: H. Thomassin, et al.; Biochim. Biophys. Acta 1137, 171 (1992), Abstract
- An affinity matrix for the purification of poly(ADP-ribose) glycohydrolase: H. Thomassin, et al.; Nucl. Acid Res. 18, 4691 (1990), Abstract
- Rapid assay of poly(ADP-ribose) glycohydrolase: L. Menard & G.G. Poirier; Biochem. Cell Biol. 65, 668 (1987), Abstract
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