PANEL of (h) TR Reporter Assays: TRα & TRβ

INDIGO’s PANEL of TR Reporter Assays utilizes non-human mammalian cells engineered to express Human Thyroid Hormone Receptors Alpha (NR1H3) and Beta (NR1H2), ligand-dependent transcription factors commonly referred to as TRα and TRβ. Because each of these reporter cells incorporates a responsive luciferase reporter gene, quantifying expressed luciferase activity provides a sensitive surrogate measure of TR activities in the treated cells. The principle application of this reporter assay system is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against human TRa and TRb.

TR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.

This TR Panel is an all-inclusive cell-based assay systems. In addition to TR Reporter Cells, this kit provides two optimized media for use during cell culture and (optionally) in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.