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Selective agonist of TLR1/TLR2 complex
Selective agonist of Toll-like receptor 1 (TLR1) complexed with TLR2. Cell permeable, water soluble synthetic cationic lipohexapeptide analog of the immunologically active N-terminal portion of bacterial lipoprotein that potently activates monocytes and macrophages. Activates/represses an array of at least 140 genes partly involved in signal transduction and regulation of the immune response.
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Product Details
| Alternative Name |
Pam3CSK4 . 3HCl, (S)-[2,3-Bis(palmitoyloxy)-(2-RS)-propyl]-N-palmitoyl-(R)-Cys-(S)-Ser-(S)-Lys4-OH . 3HCl, Pam3Cys-SKKKK |
|---|---|
| CAS |
112208-00-1 |
| Concentration |
1mg/ml after reconstitution. |
| Formula |
C81H156N10O13S . 3HCl |
| Formulation |
Lyophilized. |
| MW |
1510.3 . 109.4 |
| Quality Control |
Confirmed by ESI-MS. |
| Reconstitution |
Reconstitute with 2 ml pyrogen-free deionized water in a pyrogen-free tube. Reconstitution can also be performed in DMSO (1mg/ml stock solution). Homogenize by vortexing or use a homogenizer or sonicator to prepare a homogenous solution. Further dilutions can be made with cell culture medium or pyrogen-free deionized water. PBS is not recommended for reconstitution or dilution. |
| Solubility |
Soluble in pyrogen-free deionized water, DMSO or aqueous buffers at pH <7.5. |
| Technical Info / Product Notes |
For negative control see Prod. No. ALX-165-071 |
Handling & Storage
| Use/Stability |
As indicated on product label or CoA when stored as recommended. |
|---|---|
| Handling |
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
| Long Term Storage |
-20°C |
| Shipping |
Ambient Temperature |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Neuroinflammation, autoinflammation, splenomegaly and anemia caused by bi-allelic mutations in IRAK4: Cooray, S., Price-Kuehne, F., et al.; Front. Immunol. 14, 1231749 (2023), Abstract
- Intralesional TLR4 agonist treatment strengthens the organ defense against colonizing cancer cells in the brain: R. Blazquez, et al.; Oncogene 41, 5008 (2022), Abstract
- Role of extracellular microRNA-146a-5p in host innate immunity and bacterial sepsis: Wang, S., Yang, Y., et al.; iScience 24, 103441 (2021), Abstract
- N4BP1 negatively regulates NF-κB by binding and inhibiting NEMO oligomerization: H. Shi, et al.; Nat. Commun. 12, 1379 (2021), Abstract
- Subversion of Lipopolysaccharide Signaling in Gingival Keratinocytes via MCPIP-1 Degradation as a Novel Pathogenic Strategy of Inflammophilic Pathobionts: A. Gasiorek, et al.; Mbio 12, 502 (2021), Abstract
- Intranasal immunization with peptide-based immunogenic complex enhances BCG vaccine efficacy in a murine model of tuberculosis: Kumar, S., Bhaskar, A., et al.; JCI Insight 6, (2021), Reactant(s): Mouse, Abstract
- Activation through toll-like receptor 2 on group2 innate lymphoid cells can induce asthmatic characteristics: T. Ishii, et al.; Clin. Exp. Allergy 49, 1624 (2019), Abstract
- Monocyte procoagulant responses to anthrax peptidoglycan are reinforced by proinflammatory cytokine signaling: N.I. Popescu, et al.; Blood Adv. 3, 2436 (2019), Abstract — Full Text
- Simvastatin Inhibits Toll-like Receptor 8 (TLR8) Signaling in Primary Human Monocytes and Spontaneous Tumor Necrosis Factor Production from Rheumatoid Synovial Membrane Cultures: L. Mullen, et al.; Mol. Med. 21, 726 (2015), Abstract — Full Text
- Myeloid-specific Rictor deletion induces M1 macrophage polarization and potentiates in vivo pro-inflammatory response to lipopolysaccharide: W.T. Festuccia, et al.; PLoS One 9, e95432 (2014), Abstract — Full Text
- CD14 and TRIF govern distinct responsiveness and responses in mouse microglial TLR4 challenges by structural variants of LPS: T. Regen, et al.; Brain Behav. Immun. 25, 957 (2011), Abstract
- Pulmonary surfactant phosphatidylglycerol inhibits Mycoplasma pneumoniae-stimulated eicosanoid production from human and mouse macrophages: P. Kandasamy, et al.; J. Biol. Chem. 286, 7841 (2011), Abstract
- Delayed addition of glucocorticoids selectively suppresses cytokine production in stimulated human whole blood: D.L. Horton, et al.; Clin. Vaccine Immunol. 17, 979 (2010), Abstract — Full Text
- Toll-like receptor 2 signaling in CD4(+) T lymphocytes promotes T helper 17 responses and regulates the pathogenesis of autoimmune disease: J.M. Reynolds, et al.; Immunity 32, 692 (2010), Abstract
- TLR-mediated B cell activation results in ectopic CLIP expression that promotes B cell-dependent inflammation: M.K. Newell, et al.; J. Leukoc. Biol. 88, 779 (2010), Abstract
- Chronic pulmonary LPS tolerance induces selective immunosuppression while maintaining the neutrophilic response: S. Natarajan, et al.; Shock 33, 162 (2010), Abstract — Full Text
- Human Langerhans cells selectively activated via Toll-like receptor 2 agonists acquire migratory and CD4+T cell stimulatory capacity: M. Peiser, et al.; J. Leukoc. Biol. 83, 1118 (2008), Abstract
- The role of TLR2 in the inflammatory activation of mouse fibroblasts by human antiphospholipid antibodies: N. Satta, et al.; Blood 109, 1507 (2007), Abstract
- Intestinal alkaline phosphatase detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota: J.M. Bates, et al.; Cell Host Microbe 2, 1 (2007), Abstract — Full Text
- TLR4, but not TLR2, mediates IFN-beta-induced STAT1alpha/beta-dependent gene expression in macrophages: V. Toshchakov, et al.; Nat. Immunol. 3, 392 (2002), Abstract
- Immunostimulation by the synthetic lipopeptide P3CSK4: TLR4-independent activation of the ERK1/2 signal transduction pathway in macrophages: M.R. Muller, et al.; Immunology 103, 49 (2001), Abstract
- Adjuvant effects of various lipopeptides and interferon-gamma on the humoral immune response of chickens: M.H. Erhard, et al.; Poult. Sci. 79, 1264 (2000), Abstract
- Alteration of the lateral organization of the plasma membrane of Chinese hamster ovary cells by synthetic lipopeptide, Pam3Cys-Ser-Lys4: I. Vergne & L. Cezanne; Eur. J. Biochem. 264, 369 (1999), Abstract — Full Text
- Adjuvant lipopeptide interaction with model membranes: J. Gonzalez-Christen; Biochim. Biophys. Acta 1368, 97 (1998), Abstract
- Evidence for common mechanisms in the transcriptional control of type II nitric oxide synthase in isolated hepatocytes. Requirement of NF-kappaB activation after stimulation with bacterial cell wall products and phorbol esters: M.J. Diaz-Guerra, et al.; J. Biol. Chem. 271, 30114 (1996), Abstract — Full Text
- Lipopeptides are effective stimulators of tyrosine phosphorylation in human myeloid cells: S. Offermanns, et al.; Biochem. J. 282, 551 (1992), Abstract
- The influence of various adjuvants on antibody synthesis following immunization with an hapten: J. Kellner, et al.; Biol. Chem. Hoppe Seyler 373, 51 (1992), Abstract
- Synthesis of novel immunologically active tripalmitoyl-S-glycerylcysteinyl lipopeptides as useful intermediates for immunogen preparations: J. Metzger, et al.; Int. J. Pept. Protein Res. 37, 46 (1991), Abstract
- Activation of superoxide formation and lysozyme release in human neutrophils by the synthetic lipopeptide Pam3Cys-Ser-(Lys)4. Involvement of guanine-nucleotide-binding proteins and synergism with chemotactic peptides: R. Seifert, et al.; Biochem. J. 267, 795 (1990), Abstract
- Lipopeptide derivatives of bacterial lipoprotein constitute potent immune adjuvants combined with or covalently coupled to antigen or hapten: A. Reitermann, et al.; Biol. Chem. Hoppe Seyler 370, 343 (1989), Abstract
- Induction of tumor cytotoxicity in murine bone marrow-derived macrophages by two synthetic lipopeptide analogues: P. Hoffmann, et al.; Biol. Chem. Hoppe Seyler 370, 575 (1989), Abstract
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