p70 S6K activity kit

• Safe – non-radioactive measurement of kinase activity
• Flexible – kinetic and end-point options available
• Fast – results in < 4.5 hours
• Efficient – only 30 μl diluted sample needed per well

The p70 S6K activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.

p70 S6K is a mitogen stimulated serine/threonine protein kinase that regulates the cell growth and G1 phase cell cycle progression. p70 S6K is activated by serum stimulation and can be inhibited by both wortmannin and rapamycin. Activation of p70 S6K by multi-site phosphorylation requires phosphoinositide 3-kinase (PI3-K)-dependent signal(s) leading to sequential phosphorylation at two key residues: Thr389 and Thr229. Thr229 phosphorylation is mediated by phosphoinositide-dependent kinase 1 (PDK-1), and is dependent upon prior phosphorylation of Thr389. Multiple residues in the p70 S6K pseudosubstrate domain (e.g. Ser411, Thr421, and Ser424) are also subject to activating phosphorylation by upstream kinases.