Home Shop P-glycoprotein monoclonal antibody (C219)

P-glycoprotein monoclonal antibody (C219)

ALX-801-002

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This antibody is covered by our Worry-Free Guarantee.

ALX-801-039 MRP4 monoclonal antibody (M4I-80) Western Blot Mouse 2 — Expression of selected transporters and tight junction proteins in brain capillaries of mice exposed to bilateral renal ischemia/reperfusion injury at the protein level. Brain capillaries were isolated from frozen brains of mice exposed to bilateral renal ischemia/reperfusion injury followed by protein extraction and Western blot (A). The protein content of Bcrp (Abcg2, ATP Binding Cassette Subfamily G Member 2) (B), Glut1 (Slc2a1, Solute Carrier Family 2 Member 1) (C), Mrp4 (Abcc4, ATP Binding Cassette Subfamily C Member 4) (D), and P-gp (Mdr1, Abcb1b ATP Binding Cassette Subfamily B Member1) (E) was normalized to β-actin and is shown as average of n = 3 with standard deviations. *p < 0.05, ***p < 0.001.

Image collected and cropped by CiteAb under a CC-BY license from the following publication: Kidney Ischemia/Reperfusion Injury Induces Changes in the Drug Transporter Expression at the Blood-Brain Barrier in vivo and in vitro. Front Physiol (2020)

ALX-801-002 P-glycoprotein monoclonal antibody (C219) Western Blot Rat 1 — Biochemical characterization of rat liver subcellular fractions and Pgp localization in endolysosomal-enriched fractions. Rat liver subcellular fractionation was performed by low speed centrifugation and subsequent density gradient ultracentrifugation. Recovery and purity of the cell organelles from collected gradient fractions 1–10 (top to bottom) were analyzed by separation of equal protein amounts (5 µg) of each fraction and whole-cell homogenate (H) and post-nuclear supernatant (PNS) by SDS-PAGE and immunoblotting. Homogenate and PNS data were similar; thus, PNS is shown here for only some proteins. Endolysosomes were detected using the LAMP-2 and Rab7 marker proteins and the luminal lysosomal protease cathepsin D (CatD). EEA1 was used as a marker for early endosomes. The purity of the endolysosomal fractions was assessed by organelle markers for mitochondria (VDAC), canalicular plasma membrane (ABCB4), and ER (calnexin). (A) Representative Western blots showing the distribution of the different organelle protein markers and Pgp in the gradient fractions. Some fractions (mainly 2 and 3) contained low protein amounts and thus could not be characterized for the presence of all marker proteins for each individual gradient. Therefore, marker quantification in the gradient fractions was repeatedly performed for different fractionations. (B) Quantification of the protein band intensities for the marker proteins in the different gradient fractions. Immunoblotting revealed recovery of endolysosomal vesicles from the top of the gradient (fractions 1–4) and co-fractionation of Pgp in the enriched endolysosomal fractions (1–4). Fraction 1 was essentially free of canalicular plasma membrane contamination and mitochondria but contains remnants of ER. Mitochondria localized in fractions 7–10 and were clearly separated from endolysosomal markers. The ER marker calnexin was present in each fraction, with the lowest amount in endolysosomal fraction 1. Values are expressed as the percentage of total marker protein levels detected in all fractions combined (set to 100%). Data are presented as means ± SEM; the number of quantified Western blots (n) is shown below the graphs.

Image collected and cropped by CiteAb under a CC-BY license from the following publication: Is P-Glycoprotein Functionally Expressed in the Limiting Membrane of Endolysosomes? A Biochemical and Ultrastructural Study in the Rat Liver. Cells (2022)

ALX-801-002 P-glycoprotein monoclonal antibody (C219) Western Blot Human 1 — Expression and functional analysis of MDR1. (a, b) The expression of TUBB3 was investigated by real-time quantitative PCR and Western blotting in each cell line. Values represent mean ± SD. ∗P < 0.05, versus parental cell line. (c) Functional activity of MDR1 was analyzed by EFLUXX-ID Green Multidrug Resistance Assay. The left black curve shows unstained cells as a negative control. The right blue curve shows cells stained with EFLUXX-ID Green.

Image collected and cropped by CiteAb under a CC-BY license from the following publication: Class III β-Tubulin Overexpression Induces Chemoresistance to Eribulin in a Leiomyosarcoma Cell Line. Anal Cell Pathol (Amst) (2018)

Product Details

Alternative Name	ABCB1, CD243, P-glycoprotein 1
Application	ICC, IHC (FS), IHC (PS), IP
Application Notes	Flow Cytometry: cell permeabilization required. Detects bands of ~170kDa (MDR1 P-glycoprotein) and of ~140kDa (MDR3 P-glycoprotein) by Western blot. Cited applications include ELISA, IF, FC, and WB
Clone	C219
Crossreactivity	Cross-reacts with a ~200kDa protein which migrates in the same position as myosin and with C-erbB2 protein (p185c-erbB2).
Formulation	Phosphate-buffered solution with BSA and 0.09% NaN3
Host	Mouse
Immunogen	SDS-solubilized plasma membranes of an MDR (multidrug resistant) Chinese hamster ovary (CHO) cell line and a human cell line.
Isotype	IgG1κ
Positive Control	Cell lines: Appropriate drug-sensitive parental cell lines and their multidrug resistant derivatives prepared by the same methods as the test specimens can be used as control materials.Tissue: Human liver (positive staining detected along luminal surfaces of bile canaliculi) or human colon (positive staining localized to luminal surface of secretory epithelium) are recommended.
Recommendation Dilutions/Conditions	Immunohistochemistry (paraffin sections):- For optimal staining, the sections should be pretreated with an antigen unmasking solution.- The antibody may be diluted to ≥1:25 for biotin based detection systems.- For optimal staining, the primary antibody should be incubated 60 minutes at room temperature.Western Blot (1:50-1:100)Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Species Reactivity	Dog, Human, Mouse, Primate, Rat
Specificity	Recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, P-glycoprotein and MDR3 P-glycoprotein.
UniProt ID	P08183
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Keep at +4°C. Do not freeze.
Short Term Storage	+4°C
Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Molecular determinants of neuroprotection in blood-brain interfaces of the cynomolgus monkey: Strazielle, N., Blondel, S., et al.; Front. Pharmacol. 16, 1523819 (2025), Abstract
The Role of Elacridar, a P-gp Inhibitor, in the Re-Sensitization of PAC-Resistant Ovarian Cancer Cell Lines to Cytotoxic Drugs in 2D and 3D Cell Culture Models: Stasiak, P., Sopel, J., et al.; Int. J. Mol. Sci. 26, 1124 (2025), Abstract
Abcg2a is the functional homolog of human ABCG2 expressed at the zebrafish blood-brain barrier: Thomas, J. R., Frye, W. J. E., et al.; Fluids Barriers CNS 21, 27 (2024), Abstract
Evaluation of ABT-751, a novel anti-mitotic agent able to overcome multi-drug resistance, in melanoma cells.: Mahgoub, T. M., Jordan, E. J., et al.; Cancer Chemother. Pharmacol. 93, 427 (2024), Reactant(s): Human, Abstract
Dependence of ABCB1 transporter expression and function on distinct sphingolipids generated by ceramide synthases-2 and -6 in chemoresistant renal cancer: W.K. Lee, et al.; Int. J. Mol. Med. 31, 101492 (2022), Application(s): WB / Reactant(s) Human, Abstract
Is P-Glycoprotein Functionally Expressed in the Limiting Membrane of Endolysosomes? A Biochemical and Ultrastructural Study in the Rat Liver.: Gericke, B., Wienböker, I., et al.; Cells 11, (2022), Application(s): WB / Reactant(s): Rat, Abstract
The TH1902 Docetaxel Peptide-Drug Conjugate Inhibits Xenografts Growth of Human SORT1-Positive Ovarian and Triple-Negative Breast Cancer Stem-like Cells: Demeule, M., Charfi, C., et al.; Pharmaceutics 14, (2022), Abstract
Megalencephalic leukoencephalopathy with subcortical cysts is a developmental disorder of the gliovascular unit.: Vivien, D., Estévez, R., et al.; Elife 10, (2021), Application(s): WB, Abstract
Similarities and differences in the localization, trafficking, and function of P-glycoprotein in MDR1-EGFP-transduced rat versus human brain capillary endothelial cell lines.: Löscher, W., Noack, A., et al.; Fluids Barriers CNS 18, 36 (2021), Application(s): WB, Abstract
Quantification of P-Glycoprotein in the Gastrointestinal Tract of Humans and Rodents: Methodology, Gut Region, Sex, and Species Matter: Y. Mai, et al.; Mol. Pharm. 18, 1895 (2021), Abstract

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P-glycoprotein monoclonal antibody (C219)

ALX-801-002

Product Details

Handling & Storage

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