AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Shipping: Available products typically ship within 24/48h, via priority shipping.
Do you need support? Contact Customer Service or Technical Support.
Online Account
Access or Create Your Account
This antibody is covered by our Worry-Free Guarantee.
Product Details
| Alternative Name |
ABCB1, CD243, P-glycoprotein 1 |
|---|---|
| Application |
Flow Cytometry, FUNC, IHC (FS), IP |
| Application Notes |
Functional Application: Inhibits P-glycoprotein function. |
| Clone |
MRK16 |
| Crossreactivity |
Does not cross-react with mouse P-glycoprotein. |
| Formulation |
Liquid. In PBS containing 1% BSA and 0.1% sodium azide. |
| Host |
Mouse |
| Immunogen |
Adriamycin-resistant human myelogenous leukemia K-562/ADM cells. |
| Isotype |
IgG2a |
| Purity Detail |
Protein A affinity purified. |
| Recommendation Dilutions/Conditions |
Flow Cytometry: For 5×105 pelleted cells use 50µl of 10-100µg/ml.Immunohistochemistry (frozen sections): 0.1-10µg/ml. Use mild fixation treatment such as 4% paraformaldehyde. Do not use acetone, paraffin, methanol or ethanol as a fixative. Iodine labelling can also be performed on MRK16.Immunoprecipitation: 25-50µg/mlSuggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Species Reactivity |
Human |
| Specificity |
Recognizes an external epitope of human P-glycoprotein. Also recognizes P-glycoprotein of human cortical adenomas. |
| UniProt ID |
P08183 |
| Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
| Handling |
Avoid freeze/thaw cycles. |
|---|---|
| Long Term Storage |
-20°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Time-dependent effects of imatinib in human leukaemia cells: a kinetic NMR-profiling study: Klawitter, J., Anderson, N., et al.; Br. J. Cancer 100, 923 (2009), Abstract
- Human placental transport of vinblastine, vincristine, digoxin and progesterone: contribution of P-glycoprotein: F. Ushigome, et al.; Eur. J. Pharmacol. 408, 1 (2000), Abstract
- Functional characterization of glycosylation-deficient human P-glycoprotein using a vaccinia virus expression system: J.J. Gribar, et al.; J. Membr. Biol. 173, 203 (2000), Abstract
- Modulation of multidrug resistance in human leukemia cells with mdr1-targeted antisense oligonucleotides using variable treatment schedules: H. Dassow, et al.; Int. J. Clin. Pharmacol. Ther. 38, 209 (2000), Abstract
- Conformational heterogeneity of P-glycoprotein: K. Goda, et al.; Cancer Detect. Prev. 24, 415 (2000), Abstract
- Discordance of P-glycoprotein expression and function in acute leukemia: B. De Moerloose, et al.; Adv. Exp. Med. Biol. 457, 107 (1999), Abstract
- Choroid plexus epithelial expression of MDR1 P glycoprotein and multidrug resistance-associated protein contribute to the blood-cerebrospinal-fluid drug-permeability barrier: V.V. Rao, et al.; PNAS 96, 3900 (1999), Abstract — Full Text
- P-glycoprotein expression on normal and abnormally expanded natural killer cells and inhibition of P-glycoprotein function by cyclosporin A and its analogue, PSC833: M. Egashira, et al.; Blood 93, 599 (1999), Abstract — Full Text
- The drug efflux protein, P-glycoprotein, additionally protects drug-resistant tumor cells from multiple forms of caspase-dependent apoptosis: M.J. Smyth, et al.; PNAS 95, 7024 (1998), Abstract — Full Text
- Reference method for detection of Pgp mediated multidrug resistance in human hematological malignancies: a method validated by the laboratories of the French Drug Resistance Network: S. Huet, et al.; Cytometry 34, 248 (1998), Abstract
- Methods to detect P-glycoprotein-associated multidrug resistance in patients’ tumors: consensus recommendations: W.T. Beck, et al.; Cancer Res. 56, 3010 (1996), Abstract
- In vivo imaging and specific targeting of P-glycoprotein expression in multidrug resistant nude mice xenografts with [125I]MRK-16 monoclonal antibody: A.M. Scott, et al.; Nucl. Med. Biol. 22, 497 (1995), Abstract
- Cystic fibrosis-type mutational analysis in the ATP-binding cassette transporter signature of human P-glycoprotein MDR1: T. Hoof, et al.; J. Biol. Chem. 269, 20575 (1994), Abstract — Full Text
- Functional expression of P-glycoprotein in apical membranes of human intestinal Caco-2 cells. Kinetics of vinblastine secretion and interaction with modulators: J. Hunter, et al.; J. Biol. Chem. 268, 14991 (1993), Abstract — Full Text
- Topology of P-glycoprotein as determined by epitope mapping of MRK-16 monoclonal antibody: E. Georges, et al.; J. Biol. Chem. 268, 1792 (1993), Abstract — Full Text
- Binding properties of monoclonal antibodies recognizing external epitopes of the human MDR1 P-glycoprotein: A.H. Schinkel, et al.; Int. J. Cancer 55, 478 (1993), Abstract
- Specific targeting and killing activities of anti-P-glycoprotein monoclonal antibody MRK16 directed against intrinsically multidrug-resistant human colorectal carcinoma cell lines in the nude mouse model: T. Iwahashi, et al.; Cancer Res. 53, 5475 (1993), Abstract
- Decreased P-glycoprotein expression in multidrug-sensitive and -resistant human myeloma cells induced by the cytokine leukoregulin: C.H. Evans & P.D. Baker; Cancer Res. 52, 5893 (1992), Abstract
- Detection of multidrug-resistant protein, P-glycoprotein in childhood leukaemia and lymphoma: Y. Mizuno, et al.; Eur. J. Pediatr. 150, 416 (1991), Abstract
- Immunohistochemical detection of multidrug resistance associated P-glycoprotein in tumour and stromal cells of human cancers: D. Schlaifer, et al.; Br. J. Cancer 62, 177 (1990), Abstract
- Further characterization of the human adrenal-derived P-glycoprotein recognized by monoclonal antibody MRK 16 reacting with only human P-glycoprotein: I. Sugawara, et al.; Jpn. J. Cancer Res. 80, 1199 (1989), Abstract
- Multidrug resistance in cultured human leukemia and lymphoma cell lines detected by a monoclonal antibody, MRK16: Y. Ishida, et al.; Jpn. J. Cancer Res. 80, 1006 (1989), Abstract
- Mechanisms of multidrug resistance and implications for therapy: T. Tsuruo; Jpn. J. Cancer Res. 79, 285 (1988), Abstract
- A monoclonal antibody-Pseudomonas toxin conjugate that specifically kills multidrug-resistant cells: D.J. FitzGerald, et al.; PNAS 84, 4288 (1987), Abstract
- Functional role for the 170- to 180-kDa glycoprotein specific to drug- resistant tumor cells as revealed by monoclonal antibodies: H. Hamada and T. Tsuruo; PNAS 83, 7785 (1986), Abstract
Datasheet, Manuals, SDS & CofA
Manuals And Inserts
Certificate of Analysis
Please enter the lot number as featured on the product label
SDS
Enzo Life Science provides GHS Compliant SDS
If your language is not available please fill out the SDS request form