OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence)

• Quick ~1 hour protocol
• Highly sensitive to 10 pM
• Detects the presence of various ROS species

The OxiSelect™ ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.