OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)

• Measures total reactive oxygen species and reactive nitrogen species, including hydrogen peroxide, nitric oxide, peroxyl radical, and peroxynitrite anion
• Suitable for use with serum, plasma, urine, cell lysates or cell culture supernatants
• Detection sensitivity limit of 10 pM for DCF and 40 nM for hydrogen peroxide

The OxiSelect™ In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.