Outer membrane Protein A (ompA) (aeromonas salmonicida), (recombinant)

The interaction of bacterial and recombinant A-layer protein with murine macrophages was directed at determining the effect of A-protein on intracellular events that occur in primed macrophages. This was accomplished by measuring the cytotoxic product produced by peritoneal macrophages when exposed to A-protein coated latex beads. Thioglycolate elicited macrophages exhibited a low level of activation (18% cytotoxicity) that was significantly increased (48% cytotoxicity) in the presence of latex beads. Coating of the latex beads with each of the three A-protein products resulted in an increase of cytoxicity (mean +/- SEM) from 48% to 91%.

< 0.1 ng/µg of protein (<1EU/µg)

Lyophilized.

50.5 kDa

>98.0% as determined by Gel filtration analysis and SDS-PAGE gel.

Produced in E. coli.