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- Assay Golgi and ER dynamics in live cells
- Highly resistant to photo-bleaching, concentration quenching and photoconversion
- Identify modulators of secretory membrane traffic and organelle integrity
Compared with other commercially available dyes for labeling Golgi bodies, the green dye component of the ORGANELLE-ID-RGB® III detection reagent is more faithfully localized to the Golgi apparatus, with minimal staining of the endoplasmic reticulum. The red dye component of the reagent stains the endoplasmic reticulum with high fidelity and is specifically designed for use with green fluorescing probes. ORGANELLE-ID-RGB® III assay kit is validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line, Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS. The kit should also be suitable for identifying Golgi body and endoplasmic reticulum perturbing agents and thus can be a useful tool for examining the transport and recycling of molecules from the GA to ER in cellular secretory pathways.
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Figure 2: 3D Imaging of ER (red) and Golgi body (green) displayed relative to nucleus (blue). Three-dimensional reconstruction of the spatial relationship between the Golgi body and the endoplasmic reticulum using a structured illumination method. This was accomplished with the Apotome device from Carl Zeiss, Inc. This imaging hardware enabled creation of optical sections through the cell using a conventional wide field fluorescence microscope, for improved resolution along the optical axis. The optical sections were then used to create a 3-D reconstruction of the endoplasmic reticulum (red) and Golgi body (green), enabling display in their proper spatial context relative to the nucleus (blue).
Figure 1: ORGANELLE-ID-RGB® III kit staining of MDCK epithelial cells. The ER-ID® Red, GOLGI-ID® Green and Hoechst 33342 (blue) dyes highlight their respective subcellular targets with high dependability.
| Regulatory Status |
RUO – Research Use Only |
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