ODN M362 (TLRGRADE^®) (synthetic)

TLR, TLR9

Ligand

<0.002EU/µg (LAL test; BioWhittaker)

Lyophilized. Sterile.

8073 (ammonium salt)

Activity and endotoxin tested – TLRGRADE®.

12.2nmol (~98µg). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate).

For a 100µM stock solution, dissolve the total vial content in 122µl endotoxin-free water (included) or PBS (to order separately). To obtain optimal dissolving we recommend the following procedure:- Add 50% of the solvent and let dissolve for 10 min.- Add remaining 50% of the solvent and mix thoroughly.- Moderate warming may aid dissolving.

5’-tcgtcgtcgttcgaacgacgttgat-3’(lower case letters indicate phosphorothioate linkage).

Synthetic.

Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008).
For the inactive control compound, please see ALX-746-204.