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ODN 2006 (TLRGRADE^®) (synthetic)

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TLR9 ligand

CpG oligodeoxynucleotide (Type B) with phosphorothioate backbone. Specific ligand for human and mouse TLR9 (Toll-like receptor 9).

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Couple Target	TLR, TLR9
Couple Type	Ligand
Endotoxin Content	<0.002 EU/µg (LAL test; BioWhittaker)
Formulation	Lyophilized. Sterile.
MW	7720 (ammonium salt)
Purity Detail	Activity and endotoxin tested – TLRGRADE®.
Quantity	12.5nmol (~96µg). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate).
Reconstitution	For a 100µM stock solution, dissolve the total vial content in 125µl endotoxin-free water (included) or PBS (to order separately). To obtain optimal dissolving we recommend the following procedure:- Add 50% of the solvent and let dissolve for 10 min.- Add remaining 50% of the solvent and mix thoroughly.- Moderate warming may aid dissolving.
Sequence	5’-tcgtcgttttgtcgttttgtcgtt-3’(lower case letters indicate phosphorothioate linkage).
Source	Synthetic.
Technical Info / Product Notes	Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008). For the inactive control compound, please see Prod. No. ALX-746-206.

Use/Stability	As indicated on product label or CoA when stored as recommended. Aqueous stock solution is stable for 1 day when stored at +4°C.
Handling	Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
Long Term Storage	+4°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

T and B cell abnormalities, pneumocystis pneumonia, and chronic lymphocytic leukemia associated with an AIOLOS defect in patients: H.S. Kuehn, et al.; J. Exp. Med. 218, e20211118 (2021), Application(s): Flow Cytometry, Abstract
A Nonsense N -Terminus NFKB2 Mutation Leading to Haploinsufficiency in a Patient with a Predominantly Antibody Deficiency: Kuehn, H. S., Bernasconi, A., et al.; J. Clin. Immunol. 40, 1093 (2020), Abstract
Second Case of HOIP Deficiency Expands Clinical Features and Defines Inflammatory Transcriptome Regulated by LUBAC: Oda, H., Beck, D. B., et al.; Front. Immunol. 10, 479 (2019), Abstract
Human blood myeloid and plasmacytoid dendritic cells cross activate each other and synergize in inducing NK cell cytotoxicity: J.J.P. van Beek, et al.; Oncoimmunology 5, e1227902 (2016), Application(s): Human Dendritic Cells stimulation, Abstract — Full Text
TLR9-signaling is required for turning retinoic acid into a potent stimulator of RP105 (CD180)-mediated proliferation and IgG synthesis in human memory B cells: A. Eriksen, et al.; Cell. Immunol. 279, 87 (2012), Application(s): FLOW of human B cells, Abstract