TLR9 ligand
CpG oligodeoxynucleotide (Type B) with phosphorothioate backbone. Specific ligand for mouse TLR9 (Toll-like receptor 9).
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Figure 1: TLR9-dependent activation of mouse macrophages by ODN 1826 (TLRGRADE®) (synthetic) (Prod. No. ALX-746-002). Method: Culture of 5×105 cells per 200µl well was performed in serum-free conditions after addition of ODNs at the indicated concentrations. Cell activation was measured by IL12-p40 induction. Only CpG ODN 1826 (Prod. No. ALX-746-002), but not the corresponding GpC control ODN 1982 (Prod. No. ALX-746-201) activates wild type mouse macrophages. Activation is absolutely dependent on the presence of TLR9 as addressed with cells derived from TLR9-deficient mice. Note the logarithmic scale for the x and y-axis and the high activity of the active ODNs: max. stimulation is already achieved with 0.010-0.100nmol per 200µl well (10-100nM, corresponding to ~0.5-5ug/ml).

Figure 2: TLR9-dependent mitogenic activity of ODN 1826 (TLRGRADE®) (synthetic) (Prod. No. ALX-746-002) in mouse splenocytes. Method: Culture of 5×105 cells per 200µl well was performed in serum-free conditions after addition of ODNs at the indicated concentrations. Cell proliferation was analyzed after 72 hours with a 16 hours3H-thymidine pulse.Only CpG ODN 1826 (Prod. No. ALX-746-002), but not the corresponding GpC control ODN 1982 (Prod. No. ALX-746-201) induces proliferation of wild type mouse splenocytes. Induction of cell proliferation is absolutely dependent on the presence of TLR9 as addressed with cells derived from TLR9-deficient mice. Note the logarithmic scale for the x and y-axis and the high activity of the active ODNs: max. stimulation is already achieved with 0.010-0.100nmol per 200µl well (10-100nM, corresponding to ~0.5-5ug/ml).


Product Details
Alternative Name |
CpG-B |
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Couple Target |
TLR, TLR9 |
Couple Type |
Ligand |
Endotoxin Content |
<0.002EU/µg (LAL test; BioWhittaker) |
Formulation |
Lyophilized. Sterile. |
MW |
6383 (ammonium salt) |
Purity Detail |
Activity and endotoxin tested – TLRGRADE®. |
Quantity |
15nmol (~96µg). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate). |
Reconstitution |
For a 100µM stock solution, dissolve the total vial content in 150µl endotoxin-free water (included) or PBS (to order separately).To obtain optimal dissolving we recommend the following procedure:- Add 50% of the solvent and let dissolve for 10 min.- Add remaining 50% of the solvent and mix thoroughly.- Moderate warming may aid dissolving. |
Sequence |
5’-tccatgacgttcctgacgtt-3’ (lower case letters indicate phosphorothioate linkage). |
Source |
Synthetic. |
Technical Info / Product Notes |
Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008). |
Handling & Storage
Use/Stability |
As indicated on product label or CoA when stored as recommended. Aqueous stock solution is stable for 1 day when stored at +4°C. |
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Handling |
Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
Long Term Storage |
+4°C |
Shipping |
Ambient Temperature |
Regulatory Status |
RUO – Research Use Only |
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- Distinct effects of different adjuvants in the mouse model of allergic airway inflammation: H.S. Shin, et al.; Asian Pac. J. Allergy Immunol. 40, 111 (2022), Abstract
- Homeostatic and Pathogenic Roles of GM3 Ganglioside Molecular Species in TLR4 Signaling in Obesity: H. Kanoh, et al.; EMBO J. 39, e101732 (2020), Abstract — Full Text
- TLR9 activation via microglial glucocorticoid receptors contributes to degeneration of midbrain dopamine neurons: L. Maatouk, et al.; Nat. Commun. 9, 2450 (2018), Abstract — Full Text
- Dendritic cell inhibitory receptor 4 (DCIR4) is preferentially expressed on inflammatory and patrolling monocytes: Y. Kameda, et al.; Biochem. Biophys. Res. Commun. 480, 215 (2016), Application(s): Assay for production of interferon γ (IFN-γ), spleen cells, Abstract
- Enhancing radiosensitivity of human pulmonary adenocarcinoma cell line A549 by CpG ODN1826: R. Li, et al.; Cancer Biother. Radiopharm. 26, 69 (2011), Abstract
- The role of intermediary domain of MyD88 in cell activation and therapeutic inhibition of TLRs: M. Avbelj, et al.; J. Immunol. 187, 2394 (2011), Abstract — Full Text
- Cutting edge: the mechanism of invariant NKT cell responses to viral danger signals: A.J. Tyznik, et al.; J. Immunol. 181, 4452 (2008), Abstract — Full Text
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