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This monoclonal antibody specifically detects Oct4, a transcription factor essential for maintaining the pluripotency and self-renewal of embryonic stem cells. It is widely used in stem cell research and developmental biology.
Oct4 (Octamer-binding transcription factor 4) is a POU-domain transcription factor crucial for maintaining the undifferentiated state of embryonic stem cells. It is expressed in the inner cell mass of blastocysts and in induced pluripotent stem cells (iPSCs). Dysregulation of Oct4 has been implicated in various cancers and is used as a marker for pluripotency.
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Flow cytometric analysis of Oct4 in human embryonic stem (ES) cells. Human embryonic stem (ES) cells were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation/Permeabilization buffer (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-Oct4 monoclonal antibody, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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RUO – Research Use Only |
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