Cell permeable DNA stain that can be used for a wide range of applications
- Far-red fluorescent specific DNA dye
- Stable and highly pure
- Live, permeable and fixed cells can be analyzed
- No photobleaching effect
- No RNase treatment is required
- GFP and FITC compatible
- UV laser source is not required for excitation
- Validated for a wide range of cell densitites
- Quick and easy to use!
The NUCLEAR-ID® Red DNA Stain is a cell permeable dye, designed for use in a range of fluorescence detection technologies, in the discrimination of nucleated cells. It is resistant to photobleaching and is suitable for live-cell staining of nuclei. Also this dye provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry. Potential applications of this reagent for live-cell studies are in the determination of cellular DNA content and cell cycle distribution, for the detection of variations in growth patterns, for monitoring apoptosis, and for evaluating tumor cell behavior and suppressor gene mechanisms.
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NUCLEAR-ID Red DNA Stain requires lower concentration than competitor’s dye to visualize dsDNA.HeLa cells were grown to ~60% confluency. Cells were stained with NUCLEAR-ID Red DNA Stain at a final concentration of 4000x or 2000x or a competitor’s dye at the equivalent µM concentration at 37°C and gently washed post-staining. Cells were imaged at 15 min.

NUCLEAR-ID Red DNA Stain requires lower concentration than competitor’s dye to visualize dsDNA.HeLa cells were grown to ~60% confluency. Cells were stained with NUCLEAR-ID Red DNA Stain at a final concentration of 4000x or 2000x or with a competitor’s dye at an equivalent µM concentration at 37°C and gently washed post-staining. Cells were imaged at 15 min and 24h. Results show that 4000x NUCLEAR-ID Red DNA Stain was required for visualization of the dsDNA, while equivalent to 2000x was required for the competitor’s dye. At 24h, the competitor’s dye intensity and cell growth were dramatically reduced at the 2000x equivalent final concentration. At the same time point, 2000x of NUCLEAR-ID Red DNA Stain did not affect cell growth or fluorescent intensity. The NUCLEAR-ID Red DNA Stain shows lower cytotoxicity and requires lower concentration in live cell studies, resulting in lower costs.

Relative costs of using NUCLEAR-ID Red DNA in comparison to competitor dye in various applications: (A) Imaging (visualization), (B) Nucleated Cell Gating (flow cytometry) and (C) Live Cell Cycle analysis using flow cytometry. Dilutions can vary depending on cell strain and cell concentration.Notes:Assumes staining of a 100 µL staining volumeAssumes staining of a 500 µL cell suspension volumeAssumes a staining of 500 µL cell suspension volume




Product Details
Appearance |
Frozen liquid. |
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Application |
Flow Cytometry, Fluorescent detection |
Emission Maximum |
650nm |
Excitation Maximum |
566nm |
Purity |
≥93% (HPLC) |
Quality Control |
|
Quantity |
200µl |
Technical Info / Product Notes |
The NUCLEAR-ID® Red DNA Stain is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
Handling & Storage
Handling |
Protect from light. Avoid freeze/thaw cycles. |
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Short Term Storage |
-20°C |
Long Term Storage |
-80°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- Adipose tissue peroxisomal lipid synthesis orchestrates obesity and insulin resistance through LXR-dependent lipogenesis: Kleiboeker, B., He, A., et al.; Mol. Metab. 82, 101913 (2024), Abstract
- Mitochondria-ER contact sites expand during mitosis: Yu, F., Courjaret, R., et al.; iScience 27, 109379 (2024), Abstract
- ERK mediates interferon gamma-induced melanoma cell death: A. Champhekar, et al.; Mol. Cancer 165, s12943 (2023), Abstract
- Beta human papillomavirus 8E6 promotes alternative end joining.: Hu, C., Bugbee, T., et al.; Elife 12, (2023), Reactant(s): Human, Abstract
- Inhibiting membrane rupture with NINJ1 antibodies limits tissue injury: N. Kayagaki, et al.; Nature 618, 1072 (2023), Abstract
- Multi-dimensional-double-spiral (MDDS) inertial microfluidic platform for sperm isolation directly from the raw semen sample: H. Jeon, et al.; Sci. Rep. 12, 4212 (2022), Abstract — Full Text
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- CD200R1L is a functional evolutionary conserved activating receptor in human neutrophils: M.I.P. Ramos, et al.; J. Leukoc. Biol. 111, 367 (2022), Abstract
- Beta human papillomavirus 8E6 promotes alternative end-joining: Hu, C., Bugbee, T., et al.; bioRxiv , (2022), Reactant(s): Human
- Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis: T.P. Miettinen, et al.; Elife 11, 76664 (2022), Abstract
- Orthogonally-tunable and ER-targeting fluorophores detect avian influenza virus early infection.: Kang, T., Haque, M. M., et al.; Nat. Commun. 13, 5841 (2022), Reactant(s): Human, Abstract
- TLR2 senses the SARS-CoV-2 envelope protein to produce inflammatory cytokines: Zheng, M., Karki, R., et al.; Nat. Immunol. 22, 829 (2021), Abstract
- Subjects at-risk for future development of rheumatoid arthritis demonstrate a PAD4-and TLR-dependent enhanced histone H3 citrullination and proinflammatory cytokine production in CD14hi monocytes: Okamato, Y., Ghosh, T., et al.; J. Autoimmun. 117, 102581 (2021), Abstract
- Single-cell monitoring of dry mass and dry density reveals exocytosis of cellular dry contents in mitosis: Miettinen, T. P., Ly, K. S., et al.; bioRxiv , (2021)
- Combined epigenetic and metabolic inhibition blocks platinum-induced ovarian cancer stem cell enrichment: Sood, R., Sriramkumar, S., et al.; bioRxiv , (2021)
- Brain microvasculature has a common topology with local differences in geometry that match metabolic load: Ji, X., Ferreira, T., et al.; Neuron 109, 1168 (2021), Abstract
- Hydrogen Peroxide Is Crucial for NLRP3 Inflammasome-Mediated IL-1β Production and Cell Death in Pneumococcal Infections of Bronchial Epithelial Cells; Journal of Innate Immunity: S. Surabhi, et al.; J. Innate Immun. 10, 1159 (2021), Abstract
- DYRK1A negatively regulates CDK5-SOX2 pathway and self-renewal of glioblastoma stem cells: B. Chen, et al.; Int. J. Mol. Sci. 22, 4011 (2021), Abstract — Full Text
- Global phosphoproteomics reveals DYRK1A regulates CDK1 activity in glioblastoma cells: A. Recasens, et al.; Cell Death Discov. 7, 81 (2021), Abstract — Full Text
- Licochalcone A inhibits hypoxia-inducible factor-1α accumulation by suppressing mitochondrial respiration in hypoxic cancer cells: M.K. Park, et al.; Biomed. Pharmacother. 133, 111082 (2021), Abstract
- NINJ1 mediates plasma membrane rupture during lytic cell death: N. Kayagaki, et al.; Nature 591, 131 (2021), Abstract
- Overlap of NatA and IAP substrates implicates N-terminal acetylation in protein stabilization: F. Mueller, et al.; Sci. Adv. 7, eabc8590 (2021), Abstract — Full Text
- The SGLT-2 inhibitor empagliflozin improves myocardial strain, reduces cardiac fibrosis and pro-inflammatory cytokines in non-diabetic mice treated with doxorubicin: V. Quagliariello, et al.; Cardiovasc. Diabetol. 20, 150 (2021), Abstract — Full Text
- Therapeutic ACPA inhibits NET formation: a potential therapy for neutrophil-mediated inflammatory diseases: R.G.S. Chirivi, et al.; Cell. Mol. Immunol. 18, 1528 (2021), Abstract — Full Text
- TRIM37 is highly expressed during mitosis in CHON-002 chondrocytes cell line and is regulated by miR-223: Brigant, B., Demont, Y., et al.; Bone 137, 115393 (2020), Abstract
- A comprehensive guide to studying inflammasome activation and cell death: Tweedell, R. E., Malireddi, R. K. S., et al.; Nat. Protoc. 15, 3284 (2020), Abstract
- PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy: S. Gorelick, et al.; Elife 8, e45919 (2019), Abstract — Full Text
- Preclinical evaluation of the pan-fgfr inhibitor ly2874455 in frs2-amplified liposarcoma: R. Hanes, et al.; Cells 8, 189 (2019), Application(s): IncuCyte® image capture of dedifferentiated liposarcoma cell lines, Abstract — Full Text
- The WT1-like transcription factor Klumpfuss maintains lineage commitment of enterocyte progenitors in the Drosophila intestine.: Korzelius, J., Azami, S., et al.; Nat. Commun. 10, 4123 (2019), Reactant(s): Drosophila melanogaster, Abstract
- Altered secretion patterns and cell wall organization caused by loss of PodB function in the filamentous fungus Aspergillus nidulans: K.R. Boppidi, et al.; Sci. Rep. 8, 11433 (2018), Application(s): Fluorescence microscopy of stained fungal mycelia, Abstract — Full Text
- Gasdermin D Promotes AIM2 Inflammasome Activation and Is Required for Host Protection against Francisella novicida: Zhu, Q., Zheng, M., et al.; J. Immunol. 201, 3662 (2018), Abstract
- A high-throughput real-time imaging technique to quantify NETosis and distinguish mechanisms of cell death in human neutrophils: S. Gupta, et al.; J. Immunol. 200, 869 (2018), Application(s): IncuCyte® image capture of neutrophils, Abstract — Full Text
- Caspase-11 auto-proteolysis is crucial for noncanonical inflammasome activation: B.L. Lee, et al.; J. Exp. Med. 215, 2279 (2018), Application(s): IncuCyte® image capture of macrophages, Abstract — Full Text
- Fluorescence correlation spectroscopy on genomic DNA in living cells: C. Hodges, et al.; Methods Mol. Biol. 1814, 415 (2018), Abstract
- ZEB1-repressed microRNAs inhibit autocrine signaling that promotes vascular mimicry of breast cancer cells: E.M. Langer, et al.; Oncogene 37, 1005 (2018), Abstract — Full Text
- CDK5RAP2 interaction with components of the Hippo signaling pathway may play a role in primary microcephaly: S.K. Sukumaran, et al.; Mol. Genet. Genomics 292, 365 (2017), Application(s): Flow cytometry using primary fibroblasts, Abstract — Full Text
- Synthesis and biological evaluation of kresoxim-methyl analogues as novel inhibitors of hypoxia-inducible factor (HIF)-1 accumulation in cancer cells: S. Lee, et al.; Bioorg. Med. Chem. Lett. 27, 3026 (2017), Application(s): Confocal microscopy using HCT116 cells, Abstract
- Nesprin-2 Interacts with Condensin Component SMC2: X. Xing, et al.; Int. J. Cell Biol. 2017, 8607532 (2017), Abstract — Full Text
- A platform for brain-wide imaging and reconstruction of individual neurons.: Svoboda, K., Chandrashekar, J., et al.; Elife 5, e10566 (2016), Reactant(s): Mouse, Abstract
- How azobenzene photoswitches restore visual responses to the blind retina: I. Tochitsky, et al.; Neuron 92, 100 (2016), Application(s): Confocal microscopy using retinal ganglion cells, Abstract — Full Text
- Real-time tracking of parental histones reveals their contribution to chromatin integrity following DNA damage: S. Adam, et al.; Mol. Cell 64, 65 (2016), Application(s): Confocal microscopy using U2OS cells, Abstract — Full Text
- Plasminogen activator inhibitor-1 regulates tumor initiating cell properties in head and neck cancers: Y.C. Lee, et al.; Head Neck 38 Suppl 1, E895 (2015), Abstract
- Cellular IAP proteins and LUBAC differentially regulate necrosome-associated RIP1 ubiquitination: M.C. de Almagro, et al.; Cell Death Dis. 6, e1800 (2015), Abstract — Full Text
- Pervasive axonal transport deficits in multiple sclerosis models: C.D. Sorbara, et al.; Neuron 84, 1183 (2014), Abstract
- Zscan4 is regulated by PI3-kinase and DNA-damaging agents and directly interacts with the transcriptional repressors LSD1 and CtBP2 in mouse embryonic stem cells: M.P. Storm, et al.; PLoS One 9, e89821 (2014), Application(s): Flow cytometry measurements on mouse pluripotent cells, Abstract — Full Text
- Cellular, subcellular and functional in vivo labeling of the spinal cord using vital dyes: Romanelli, E., Sorbara, C. D., et al.; Nat. Protoc. 8, 481 (2013), Abstract
- Epigenetic regulation of planarian stem cells by the SET1/MLL family of histone methyltransferases: A. Hubert, et al.; Epigenetics 8, 79 (2013), Application(s): Cell cycle analysis by flow cytometry, Abstract — Full Text
- Wntless is required for peripheral lung differentiation and pulmonary vascular development: B. Cornett, et al.; Dev. Biol. 379, 38 (2013), Application(s): Detection of nuclei in lung cells by flow cytometry, Abstract
- Ionizing radiation induces mitochondrial reactive oxygen species production accompanied by upregulation of mitochondrial electron transport chain function and mitochondrial content under control of the cell cycle checkpoint: T. Yamamori, et al.; Free Radic. Biol. Med. 53, 260 (2012), Application(s): Cell cycle analysis by flow cytometry, Abstract
- Neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle Formation in HEK293 Cells: P. Chen, et al.; PLoS One 7, e45322 (2012), Application(s): DNA nuclei staining by flow cytometry, Abstract — Full Text
- Synthesis, cytotoxicity and cellular uptake studies of N3 functionalized Re(CO)3 thymidine complexes: M.D. Bartholomä, et al.; Dalton Trans. 40, 6216 (2011), Application(s): Nuclear DNA stain of human lung adenocarcinoma cells, Abstract
- A cell-permeant dye for cell cycle analysis by flow and laser-scanning microplate cytometry: Y.J. Xiang, et al.; Nat. Methods 6, an2 (2009), Application(s): Cell cycle analysis by flow cytometry, Abstract
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