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Nuclear extract from HeLa cells

BML-KI140

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Prepared by high salt extraction of HeLa nuclei (human cervical cancer cell line), this extract is a rich source of HDAC activity, useful for inhibitor screening or immunoprecipitation of HDAC containing complexes. Typically, 0.5µl or less per well is sufficient for assay with the HDAC FLUOR DE LYS^® fluorometric activity assay (Prod. No. BML-AK500).

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Product Details

Formulation	Liquid. In 0.1M KCl, 20mM HEPES/NaOH, pH 7.9, 20% (v/v) glycerol, 0.2mM EDTA, 0.5mM DTT, 0.5mM PMSF.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Epigenetic Histone Deacetylases Activity Assay in the Brain and Peripheral Organ Tissues: Reddy, D. S., Boyd, H., et al.; Curr. Protoc. 5, e70143 (2025), Abstract
Synthesis and biological evaluation of ortho-phenyl phenylhydroxamic acids containing phenothiazine with improved selectivity for class IIa histone deacetylases: Hsu, K. C., Huang, Y. Y., et al.; J. Enzyme Inhib. Med. Chem. 39, 2406025 (2024), Abstract
Discovery of Novel Src Homology-2 Domain-Containing Phosphatase 2 and Histone Deacetylase Dual Inhibitors with Potent Antitumor Efficacy and Enhanced Antitumor Immunity: M. Liu, et al.; J. Med. Chem. 65, 12200 (2022), Abstract
Measuring Histone Deacetylase Inhibition in the Brain: D.S. Reddy, et al.; Curr. Protoc. Pharmacol. 81, e41 (2018), Reactant(s) Mouse, Abstract
Design, synthesis and evaluation of novel N-hydroxybenzamides/N-hydroxypropenamides incorporating quinazolin-4(3H)-ones as histone deacetylase inhibitors and antitumor agents: D.T. Hieu, et al.; Bioorg. Chem. 76, 258 (2017), Abstract
Biphenyl-4-yl-acrylohydroxamic acids: Identification of a novel indolyl-substituted HDAC inhibitor with antitumor activity: R. Cincinelli, et al.; Eur. J. Med. Chem. 112, 99 (2016), Application(s): Compounds were tested for HDAC inhibition on HeLa nuclear extract, Abstract
Synthesis and evaluation of aliphatic-chain hydroxamates capped with osthole derivatives as histone deacetylase inhibitors: W.H. Huang, et al.; Eur. J. Med. Chem. 46, 4042 (2011), Abstract

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Nuclear extract from HeLa cells

BML-KI140

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

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Nuclear extract from HeLa cells

BML-KI140

Product Details

Handling & Storage

Related Products

FLUOR DE LYS® HDAC fluorometric activity assay kit

BML-AK500

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

FLUOR DE LYS^® HDAC fluorometric activity assay kit