Nuclear/Cytosolic Fractionation Kit

• Simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells
• Extraction in less than 2 hours
• Each prep can accommodate up to 5 x 106cells

Cell Biolabs’ Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells.  The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours.  The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting.