Home Shop Nitric oxide synthase (universal) monoclonal antibody (NOS-3F7-B11-B5)

Nitric oxide synthase (universal) monoclonal antibody (NOS-3F7-B11-B5)

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Alternative Name	NOS (universal)
Application	IHC (FS), WB
Application Notes	Detects bands of ~130kDa (representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS)), ~155kDa (in tissues expressing nNOS) and ~140kDa (in tissues expressing eNOS) by Western blot.
Clone	NOS-3F7-B11-B5
Formulation	Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
Host	Mouse
Immunogen	Purified neuronal nitric oxide synthase (nNOS; NOS I) from bovine brain.
Isotype	IgM
Recommendation Dilutions/Conditions	Immunohistochemistry (frozen sections, 1:100)Western Blot (1:50-1:500)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Bovine, Mouse, Rat
Specificity	Recognizes iNOS (NOS II), eNOS (NOS III) and nNOS (NOS I).
UniProt ID	E1BKI3
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Regulatory Status	RUO – Research Use Only

Multiple distinct subtypes of GABAergic neurons in mouse visual cortex identified by triple immunostaining.: Gonchar, Y., Burkhalter, A., et al.; Front. Neuroanat. 1, 3 (2008), Application(s): IHC / Reactant(s): Mouse, Abstract
Nitric oxide protects cardiac sarcolemmal membrane enzyme function and ion active transport against ischemia-induced inactivation: K.Y. Xu, et al.; J. Biol. Chem. 278, 41798 (2003), Abstract — Full Text
Distribution of NOS in normoxic vs. hypoxic rat lung: upregulation of NOS by chronic hypoxia: C. Xue, et al.; Am. J. Physiol. 267, L667 (1994), Abstract

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