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NGAL (rat) ELISA kit

BPD-KIT-046

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Ready-to-use – pre-coated plate and individual calibrators save time and reduce errors
Fast – results from up to 40 samples in duplicate in just 4 hours per 96 well plate

A colorimetric immunometric ELISA kit for the rapid measurement of NGAL in rat samples. Absorbance is read at 450nm.

NGAL (also known as Lipocalin-2 or Neutrophil Gelatinase-associated Lipocalin) is a small, ~25kDa secreted protein expressed in epithelial tissues throughout the body.

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Product Details

Alternative Name	Neutrophil gelatinase-associated lipocalin, Lipocalin 2, Siderocalin, Oncogene 24p3
Application	Colorimetric detection, ELISA
Application Notes	For the quantitative determination of NGAL in rat urine, plasma, serum, tissue extracts, or culture supernatants.
Assay Time	4 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	1: Microwell plate coated with anti-NGAL (rat) antibody (96 wells) 2: 5X Sample Diluent 3: NGAL Rat Calibrators 1-8. 4: 25X Wash Solution Concentrate 5: Biotinylated Rat NGAL Antibody 6: HRP-Streptavidin 7: TMB Substrate 8: Stop Solution
Sensitivity	0.5pg/ml (range 4-400pg/ml)
Species Reactivity	Rat
Technical Info / Product Notes	For the manual please click here.
UniProt ID	P30152
Wavelength	450 nm

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Isoliquiritigenin Prevents the Development of Nephropathy by an HFD in Rats Through the Induction of Antioxidant Production and Inhibition of the MD-2/TLR4/NF-κB Pathway: Yahya, M. A., Alshammari, G. M., et al.; Biology (Basel) 13, (2024), Abstract
Amphora algae with low-level ionizing radiation exposure ameliorate D-galactosamine-induced inflammatory impairment in rat kidney: El-Sonbaty, S. M., Moawed, F. S. M., et al.; Environ. Toxicol. 36, 451 (2021), Abstract
Differences in the association between behavior and neutrophil gelatinase-associated lipocalin in male and female rats after coronary artery ligation: L. Gouweleeuw, et al.; Physiol. Behav. 163, 7 (2016), Abstract
Effects of obesity and sex on antimicrobial pharmacokinetics and acute kidney injury: validation of a preclinical model: M.P. Pai, et al.; Antimicrob. Agents Chemother. 57, 716 (2013), Abstract
Serum Lipocalin2 is a potential biomarker of liver irradiation damage: S. Sultan, et al.; Liver Int. 33, 459 (2013), Abstract
Phosphodiesterase-5 inhibition attenuates early renal ischemia-reperfusion-induced acute kidney injury: assessment by quantitative measurement of urinary NGAL and KIM-1: R. Sohotnik, et al.; Am. J. Physiol. Renal Physiol. 304, F1099 (2013), Abstract
Treatment with the vascular disrupting agent combretastatin is associated with impaired AQP2 trafficking and increased urine output: A.B. Bohn, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 303, R186 (2012), Abstract
Angiotensin-(1-7) attenuates diabetic nephropathy in Zucker diabetic fatty rats: J.F. Giani, et al.; Am. J. Physiol. Renal Physiol. 302, F1606 (2012), Abstract
Urinary aminopeptidase activities as early and predictive biomarkers of renal dysfunction in cisplatin-treated rats: A. Quesada, et al.; PLoS One 7, e40402 (2012), Abstract
Role of peroxynitrite in sepsis-induced acute kidney injury in an experimental model of sepsis in rats: M. Seija, et al.; Shock 38, 403 (2012), Abstract

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NGAL (rat) ELISA kit

BPD-KIT-046

Product Details

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Datasheet, Manuals, SDS & CofA

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