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NGAL (pig) ELISA kit

BPD-KIT-044

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Ready-to-use – pre-coated plate and individual calibrators save time and reduce errors
Easy-to-use – convenient storage at 4°C with room temperature incubations

A colorimetric immunometric ELISA kit for the rapid measurement of NGAL in pig samples. Absorbance is read at 450nm.

NGAL (also known as Lipocalin-2 or Neutrophil Gelatinase-associated Lipocalin) is a small, ~25kDa secreted protein expressed in epithelial tissues throughout the body.

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Product Details

Alternative Name	Neutrophil gelatinase-associated lipocalin, Lipocalin 2, Siderocalin, Oncogene 24p3
Application	Colorimetric detection, ELISA
Application Notes	For the quantitative determination of NGAL in pig urine, plasma, serum, tissue extracts, or culture supernatants.
Assay Time	4 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	1: Microwell plate (96 precoated wells); 2: 50ml 5x Sample Diluent Conc.; 3: 7 x 1ml Pig NGAL Calibrator (1-7. 0, 10, 20, 40, 100, 200, 400 pg/mL); 4: 40ml 25x Wash Solution Conc.; 5: 12ml Biotinylated Pig-NGAL Antibody; 6: 12ml HRP-Streptavidin; 7:12ml TMB Substrate; and 8: 12ml Stop Solution.
Sensitivity	1pg/ml (range 10-400pg/ml)
Species Reactivity	Porcine
Technical Info / Product Notes	For the manual please click here.
Wavelength	450 nm

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Furosemide attenuates tubulointerstitial injury and allows functional testing of porcine kidneys during normothermic machine perfusion: B. Ogurlu, et al.; Artif. Organs 48, 595 (2024), Abstract
Normothermic machine perfusion of ischaemically damaged porcine kidneys with autologous, allogeneic porcine and human red blood cells: M.B.F. Pool, et al.; PLoS One 15, e0229566 (2020), Abstract — Full Text
Remote Ischemic Preconditioning May Attenuate Renal Ischemia-Reperfusion Injury in a Porcine Model of Supraceliac Aortic Cross-Clamping: Athanasiadis, D., Kapelouzou, A., et al.; J. Vasc. Res. 52, 161 (2016), Abstract
Carbamylated erythropoietin-FC fusion protein and recombinant human erythropoietin during porcine kidney ischemia/reperfusion injury: Š. Mat&ecaron;jková, et al.; Intensive Care Med. 39, 497 (2013), Abstract
Neutrophil gelatinase-associated lipocalin (NGAL) levels in response to unilateral renal ischaemia in a novel pilot two-kidney porcine model: J.L. Silberstein, et al.; BJU Int. 112, 517 (2013), Abstract
Early outcome in renal transplantation from large donors to small and size-matched recipients – a porcine experimental model: K. Ravlo, et al.; Pediatr. Transplant. 16, 599 (2012), Abstract
Early urinary biomarkers of warm and cold ischemic injury in an experimental kidney model: S.A. Hosgood, et al.; J. Surg. Res. 174, e85 (2012), Abstract
NGAL excretion is higher from the healthy side than from the injured side in unilateral acute kidney injury: S.S. Pedersen, et al.; Scand J. Clin. Lab Invest. 72, 510 (2012), Abstract
Protective effect of low potassium dextran solution on acute kidney injury following acute lung injury induced by oleic acid in piglets: R.P. Wu, et al.; Chin. Med. J. (Engl.) 125, 3093 (2012), Abstract
Circulating AST, H-FABP, and NGAL are early and accurate biomarkers of graft injury and dysfunction in a preclinical model of kidney transplantation: I. Jochmans, et al.; Ann. Surg. 254, 784–91; discussion 791-2 (2011), Abstract

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NGAL (pig) ELISA kit

BPD-KIT-044

Product Details

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Datasheet, Manuals, SDS & CofA

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