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NF-κB (p50) (human), (recombinant) (His-tag)

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Concentration	0.2mg/ml
Formulation	Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol.
Purity	≥95% (SDS-PAGE)
Source	Produced in SF21 insect cells. Fused to a His-tag. Produced in a baculovirus expression system.
Specific Activity	~1ng is required for high mobility shift assay. ~5ng are required for reconstituted transcription assays.
UniProt ID	P19838

Handling	Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen.
Short Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

An exonuclease protection and CRISPR/Cas12a integrated biosensor for the turn-on detection of transcription factors in cancer cells: B. Li, et al.; Anal. Chim. Acta 1165, 338478 (2021), Abstract
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription: P.O. Hassa, et al.; J. Biol. Chem. 280, 40450 (2005), Abstract — Full Text
Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1: P.O. Hassa, et al.; J. Biol. Chem. 278, 45145 (2003), Abstract — Full Text

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