NF-ATc2 monoclonal antibody (25A10.D6.D2)

NFATc2, Nuclear factor of activated T cells, cytoplasmic 2, NFATP, NFAT1

Gel Supershift, ICC, IF, IHC (FS), IHC (PS), IP, WB

Detects bands of ~140kDa (phosphorylated NF-ATc2 in resting immune cells) and ~120kDa (fully-dephosphorylated NF-ATc2 in stimulated cells) by Western blot.

25A10.D6.D2

Does not cross-react with NF-ATc1.

Liquid. Purified antibody in PBS containing 0.05% sodium azide.

Mouse

Synthetic peptide corresponding to aa 51-69 (A51ISSPSGLAYPDDVLDYGL69) of mouse nuclear factor of activated T cells, cytoplasmic 2 (NF-ATc2) conserved in all three isoforms A, B and C.

IgG1

Immunocytochemistry (1:100 – 1:1,000)Immunofluorescence (1:100 – 1:1,000)Immunohistochemistry (frozen sections and paraffin sections, 1:50 – 1:200)Western Blot (1µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Human, Mouse, Rat

Note: Confusion may arise regarding NFAT protein versus gene names. According to UniProt, the nomenclature is as follows:Nuclear Factor of Activated T Cells, Cytoplasmic 1 (NF-ATc1)Gene Names: NFATC1 or NFATC or NFAT2
Nuclear Factor of Activated T Cells, Cytoplasmic 2 (NF-ATc2)Gene Names: NFATC2 or NFATP or NFAT1
Nuclear Factor of Activated T Cells, Cytoplasmic 3 (NF-ATc3)Gene Names: NFATC3 or NFATX or NFAT4
Nuclear Factor of Activated T Cells, Cytoplasmic 4 (NF-ATc4)Gene Names: NFATC4 or NFAT3
Nuclear Factor of Activated T Cells (NF-AT5)Gene Names: NFAT5

Q60591

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