Home Shop Neutrophil elastase (human), (purified)

Neutrophil elastase (human), (purified)

BML-SE284

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Major inflammatory protease released by neutrophils

Functions in inflammation. Can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α , and α 1- antichymotrypsin. Because of its potential roles in inflammatory diseases such as emphysema and cystic fibrosis, it is a target for drug discovery.

Neutrophil elastase, a serine protease found in polymorphonuclear neutrophils (PMNs), functions in inflammation.

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Product Details

Alternative Name	Leukocyte elastase, LE
Application Notes	Useful for inhibitor screening, kinetic studies, and cleavage of substrates in vitro or in tissue culture.
Purity	≥95% (SDS-PAGE). Although this is in highly purified form, and the source material tested negative for HbsAg, anti-HBc, anti-HCV, and anti-HIV-1 and -2, handle this product with all due precautions.
Reconstitution	Reconstitute with 50mM sodium acetate, pH 5.5, with 150mM sodium chloride to the original volume, followed by gentle mixing to ensure adequate homogenization.
Source	Isolated from human neutrophils. Active enzyme.
Specific Activity	20-22 U/mg protein. One unit will hydrolyze 1.0 μmole of MeOSuc-Ala-Ala-Pro-Val-pNA (Prod. No. BML-P213) per minute, at 25°C in 100mM Tris-HCl, pH 7.5 with 500 mM NaCl.
UniProt ID	P08246

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended. This enzyme is stable when stored under the above conditions. Procedures such as dilution of the enzyme followed by refreezing could lead to loss of activity.
Handling	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Long Term Storage	-20°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Chemical Optimization of Selective Pseudomonas aeruginosa LasB Elastase Inhibitors and Their Impact on LasB-Mediated Activation of IL-1β in Cellular and Animal Infection Models: M.J. Everett, et al.; ACS Infect. Dis. 9, 270 (2022), Abstract
A topological refactoring design strategy yields highly stable granulopoietic proteins: Skokowa, J., Hernandez Alvarez, B., et al.; Nat. Commun. 13, 2948 (2022), Abstract
Elastase Inhibitor Cyclotheonellazole A: Total Synthesis and In Vivo Biological Evaluation for Acute Lung Injury: Cui, Y., Zhang, M., et al.; J. Med. Chem. 65, 2971 (2022), Abstract
Multiple modes of action mediate the therapeutic effect of IVIg in experimental epidermolysis bullosa acquisita: E. Pipi, et al.; J. Invest. Dermatol. , (2021), Abstract
Design of novel granulopoietic proteins by topological rescaffolding: Hernandez Alvarez, B., Skokowa, J., et al.; PLoS Biol. 18, e3000919 (2020), Abstract
The Coagulation and Immune Systems Are Directly Linked through the Activation of Interleukin-1α by Thrombin: L.C. Burzynski, et al.; Immunity 50, 1033 (2019), Abstract
Amblyomma americanum serpin 27 (AAS27) is a tick salivary anti-inflammatory protein secreted into the host during feeding: L. Tirloni, et al.; PLoS Negl. Trop. Dis. 13, e0007660 (2019), Abstract — Full Text
Competitive neutrophil elastase inhibitory isoflavones from the roots of Flemingia philippinensis: J. Y. Kim, et al.; Bioorg. Chem. 78, 249 (2018), Abstract
Calcium-induced Tetramerization and Zinc Chelation Shield Human Calprotectin fromDegradation by Host and Bacterial Extracellular Proteases: J.R. Stephan & E.M. Nolan; Chem. Sci. 2016, 1962 (2016), Application(s): Protease digestion assays, Abstract — Full Text
MrkD1P from Klebsiella pneumoniae strain IA565 allows for coexistence with Pseudomonas aeruginosa and protection from protease-mediated biofilm detachment: B.M. Childers, et al.; Infect. Immun. 81, 4112 (2013), Abstract — Full Text

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Purity	≥98%

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Neutrophil elastase (human), (purified)

BML-SE284

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

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