Nephrin (Ig-like domain 5) clonal antibody (Y17-R)

Renal glomerulus-specific cell adhesion receptor

ELISA, IHC (PS), WB

Y17-R

Liquid. In 20mM TRIS/HCl, pH 8.0, containing 10mg/ml BSA and 0.05% sodium azide.

Rabbit

Synthetic peptide corresponding to an epitope in the centre of the fifth Ig-like domain of human nephrin (between aa 465 and 528).

IgG

Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide.

ELISA (1:50,000-1:100,000)Immunohistochemistry (paraffin sections, 1:200)Western Blot (1:5,000; Wash buffer: 1x TRIS buffered saline (TBS) containing 0.1% Triton X-100, Blocking buffer: 1x TBS containing 0.1% Triton X-100 and 5% nonfat dry milk. Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Human, Mouse, Rat

Clonal Rabbit Antibody is a pure homogenous fraction of rabbit immunoglobulin (IgG) which corresponds to a unique linear epitope on the target protein. This epitope is designed after a detailed analysis of the antigen structure, regulatory post-translational modifications, protein/protein interaction domain and protein folding. The chosen sequence (peptide) is synthesized and the rabbit is immunized. The clone is then separated from the crude antiserum by “in vitro cloning technology”. The resulting product is monospecific and characterized by superior specificity, affinity and avidity.

O60500

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