Home Shop Necrox-5™ . hydrochloride

Necrox-5™ . hydrochloride

ALX-430-247

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Necrosis inhibitor

Cell permeable necrosis inhibitor with antioxidant activity. It localizes mostly in the mitochondria. Selectively blocks oxidative stress-induced necrotic cell death (0.1μM NecroX™-5 prevented ~50% cell death in H9C2 cells exposed to 400μM t-BuOOH for 2 hours LDH assay used). Does not protect against staurosporine or etoposide-induced apoptosis. Protects cells against cold shock, hypoxia and oxidative stress in vitro, as well as CCl₄ -induced acute liver injury and chronic liver fibrosis in rodent models.

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Product Details

Alternative Name	[5-(1,1-Dioxo-thiomorpholin-4-ylmethyl)-2-phenyl-1H-indol-7-yl]-(tetrahydro-pyran-4-ylmethyl)-amine
Appearance	Yellow solid.
Application Notes	Can be used for in vitro and in vivo applications. For in vitro experiments, dissolve Necrox-5™ in DMSO to 20-50mM stock solution and dilute further in cell culture media. Treat cells with 30μM to 0.1μM final concentration. For in vivo experiments, dissolve the product in DMSO at a concentration of 10mg/ml stock. Do not use PBS and Water! Optimal conditions must be determined individually for each application.
Formula	C₂₅H₃₁N₃O₃S . 2 HCl
Identity	Determined by LC-MS.
MW	453.6 . 72.9
Purity	≥96% (HPLC, TLC)
Solubility	Soluble in DMSO (>10mg/ml).

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Protect from light. Keep under inert gas.
Long Term Storage	-20°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

Induction of Lysosomal Membrane Permeabilization Is a Major Event of FTY720-Mediated Non-Apoptotic Cell Death in Human Glioma Cells: K. Min, et al.; Cancers (Basel) 12, 3388 (2020), Abstract
Necrosis triggered byi>Mycobacterium tuberculosis/i>alters macrophage triglyceride metabolism and inflammatory response in a DGAT1 – dependent manner: Jaisinghani, N., Dawa, S., et al.; bioRxiv , (2017)
Phosphoinositide 3-kinase-δ regulates fungus-induced allergic lung inflammation through endoplasmic reticulum stress: Lee, K. S., Jeong, J. S., et al.; Thorax 71, 52 (2016), Abstract
Dysfunctional HIV-specific CD8+ T cell proliferation is associated with increased caspase-8 activity and mediated by necroptosis: Gaiha, G. D., McKim, K. J., et al.; Immunity 41, 1001 (2014), Abstract
NLRP3 inflammasome activation by mitochondrial ROS in bronchial epithelial cells is required for allergic inflammation: Kim, S. R., Kim, D. I., et al.; Cell Death Dis. 5, e1498 (2014), Abstract
Necrotic cell death in atherosclerosis: W. Martinet, et al.; Basic Res. Cardiol. 106, 749 (2011), Abstract
NecroX as a novel class of mitochondrial reactive oxygen species and ONOO(-) scavenger: H.J. Kim, et al.; Arch. Pharm. Res. 33, 1813 (2010), Abstract