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NANOG is a transcription factor critical for maintaining the pluripotency of embryonic stem cells. It is frequently used as a marker for stemness in developmental and cancer research.
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Immunofluorescence staining of nanog in hESC-H9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human nanog polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus.
| Regulatory Status |
RUO – Research Use Only |
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Last modified: June 6, 2025