NADP-NADPH Assay

NADP and NADPH play critical roles in metabolism and redox reactions. NADP acts as a coenzyme in various enzymatic reactions, particularly photosynthesis and oxidative stress defense. NADPH, the reduced form of NADP, is essential for biosynthetic reactions, such as fatty acid and nucleotide synthesis. Moreover, NADPH serves as a potent reducing agent, protecting cells from oxidative damage and maintaining redox homeostasis. The dynamic interplay between NADP and NADPH is indispensable for cellular function and overall metabolic balance. AkrivisBio’s NADP/NADPH Assay is a simple, sensitive colorimetric method for measuring NADP and/or NADPH as low as 100 picomole levels in a wide range of biological samples. In this assay, NADP in the sample is used by an NADP specific alcohol dehydrogenase to form NADPH, which then reduces a nearly colorless tetrazolium salt to a highly colored formazan measured at 450nm. The assay does not recognize NAD+/NADH.