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Epitope tagging is a widely accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, gene expression can be easily monitored on Western blotting, immunoprecipitation and immunofluorescence utilizing an antibody that recognizes such an epitope. The Myc-Tag (EQKLISEEDL) corresponds to the partial peptide sequence of the human c-myc gene product.
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Western blot analysis of 293T cells transfected with 6Myc/Heparanase (lane 1) and 6Myc/cdc25 (lane 2), probed with Myc-tag mAb (PL14).
Immunocytochemistry analysis of monkey Cos-7 cells transfected with 6myc-hCAP1, stained with Myc-tag mAb (PL14).
| Regulatory Status |
RUO – Research Use Only |
|---|
Related Products
| Application | ELISA, IHC, WB |
|---|---|
| Host | Goat |
| Species Reactivity | Mouse |