Home Shop Muscle actin monoclonal antibody (HHF35) (Ready-to-Use)

Muscle actin monoclonal antibody (HHF35) (Ready-to-Use)

ENZ-30931

9 citations

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This antibody is covered by our Worry-Free Guarantee.

Product Details

Application	IHC (PS), WB
Clone	HHF35
Formulation	Liquid. Ready-to-use solution in PBS containing 0.1% sodium azide and 1% BSA.
Host	Mouse
Immunogen	Human myocardium (proteins extracted in 2% SDS).
Isotype	IgG1
Purity Detail	Affinity purified.
Recommendation Dilutions/Conditions	Immunohistochemistry (paraffin sections; this antibody can be used for staining of paraffin-embedded tissue sections fixed in neutral buffered formalin and is compatible with most commonly used histological fixatives. Choose a good quality enzyme-linked anti-mouse IgG1 antibody for detection)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from hybridoma tissue culture supernatant.
Species Reactivity	Human, Monkey, Rabbit
Specificity	Recognizes actin isotypes α and γ of skeletal, cardiac and smooth muscle. This antibody labels rhabdomyosarcomas, hemangiopericytomas, leiomyosarcomas and leiomyomas but does not react with non-myogenic sarcomas. It is nonreactive with other neoplasms such as carcinoma or lymphoma and with other mesenchymal and all epithelial cells, with the exception of myoepithelium.
UniProt ID	P68133 (ACTA1), P63267 (ACTG2)
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Overexpression of Gremlin-1 in patients with Loeys-Dietz syndrome: implications on pathophysiology and early disease detection.: Streichert, T., Bentzien, F., et al.; PLoS One 9, e104742 (2014), Application(s): IHC / Reactant(s): Human, Abstract
Sublytic C5b-9 induces proliferation of human aortic smooth muscle cells: Role of mitogen activated protein kinase and phosphatidylinositol 3-kinase: F. Niculescu, et al.; Atherosclerosis 142, 47 (1999), Application(s): IHC on primary human ASMC cultures , Abstract
Proliferation of smooth muscle cells in acute allograft vascular rejection: Amano, J., Ishiyama, S., et al.; J. Thorac. Cardiovasc. Surg. 113, 19 (1997), Abstract
Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins: R.A. Schmidt, et al.; Am. J. Pathol. 131, 19 (1988), Abstract — Full Text
Diagnosis of Rhabdomyosarcomas using HHF35, an anti-muscle actin monoclonal antibody: R.A. Schmidt, et al.; Lab. Invest. 56, 70A (1987)
HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization: T. Tsukada, et al.; Am. J. Pathol. 126, 51 (1987), Abstract — Full Text
HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues: T. Tsukada, et al.; Am. J. Pathol. 127, 389 (1987), Abstract — Full Text
Human atherosclerosis. II. Immunocytochemical analysis of the cellular composition of human atherosclerotic lesions: A.M. Gown, et al.; Am. J. Pathol. 125, 191 (1986), Abstract — Full Text
CGA-7 and HHF, two monoclonal antibodies that recognize muscle actin and react with adherent cells in human long-term bone marrow cultures: P. Charbord, et al.; Blood 66, 1138 (1985), Abstract — Full Text

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Muscle actin monoclonal antibody (HHF35)

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Application	IHC (PS), WB
Host	Mouse
Isotype	IgG1
Species Reactivity	Human, Monkey, Rabbit

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Muscle actin monoclonal antibody (HHF35) (Ready-to-Use)

ENZ-30931

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

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