AMPIVIEW® RNA probes
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This antibody is covered by our Worry-Free Guarantee.
Product Details
| Application |
IHC (PS), WB |
|---|---|
| Clone |
HHF35 |
| Formulation |
Liquid. In PBS containing 0.1% sodium azide and 1% BSA. |
| Host |
Mouse |
| Immunogen |
Human myocardium (proteins extracted in 2% SDS). |
| Isotype |
IgG1 |
| Purity Detail |
Affinity purified. |
| Recommendation Dilutions/Conditions |
Immunohistochemistry (paraffin sections; this antibody can be used for staining of paraffin-embedded tissue sections fixed in neutral buffered formalin and is compatible with most commonly used histological fixatives. Choose a good quality enzyme-linked anti-mouse IgG1 antibody for detection).General suggested starting dilution is 1:20-1:50.Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Source |
Purified from hybridoma tissue culture supernatant. |
| Species Reactivity |
Human, Monkey, Rabbit |
| Specificity |
Recognizes actin isotypes α and γ of skeletal, cardiac and smooth muscle. This antibody labels rhabdomyosarcomas, hemangiopericytomas, leiomyosarcomas and leiomyomas but does not react with non-myogenic sarcomas. It is nonreactive with other neoplasms such as carcinoma or lymphoma and with other mesenchymal and all epithelial cells, with the exception of myoepithelium. |
| Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
| Handling |
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
|---|---|
| Short Term Storage |
+4°C |
| Long Term Storage |
-20°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Effects of withdrawing an atherogenic diet on the atherosclerotic plaque in rabbits.: Zhao, L., Li, S., et al.; Exp. Ther. Med. 22, 751 (2021), Application(s): IHC, Abstract
- Near-Infrared Autofluorescence in Atherosclerosis Associates With Ceroid and Is Generated by Oxidized Lipid-Induced Oxidative Stress.: Tearney, G. J., Libby, P., et al.; Arterioscler. Thromb. Vasc. Biol. 41, e385 (2021), Reactant(s): Human, Abstract
- Chronic Staphylococcus aureus Superantigen Toxic Shock Syndrome Toxin-1 Exposure Accelerates the Progression of Atherosclerosis in Rabbits: Zhao, H., Chen, L., et al.; Acta. Cardiol. Sin. 36, 24 (2020), Abstract
- Dynamin-Related Protein 1 Inhibition Attenuates Cardiovascular Calcification in the Presence of Oxidative Stress: Rogers, M. A., Maldonado, N., et al.; Circ. Res. 121, 220 (2017), Abstract
- Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling.: Libby, P., Sukhova, G. K., et al.; PLoS One 11, e0152474 (2016), Application(s): IHC / Reactant(s): Human, Abstract
- Coincidence between malignant perivascular epithelioid cell tumor arising in the gastric serosa and lung adenocarcinoma: S. Yamada, et al.; World J. Gastroenterol. 21, 1349 (2015), Application(s): Immunohistochemistry, Abstract
- Skeletal muscle expression of the adhesion-GPCR CD97: CD97 deletion induces an abnormal structure of the sarcoplasmatic reticulum but does not impair skeletal muscle function: Zyryanova, T., Schneider, R., et al.; PLoS One 9, e100513 (2014), Abstract
- Multiple sites of highly amplified DNA sequences detected by molecular cytogenetic analysis in HS-RMS-2, a new pleomorphic rhabdomyosarcoma cell line: Takaoka, E., Sonobe, H., et al.; Am. J. Cancer Res. 2, 141 (2011), Abstract
- L1 (CD171) is highly expressed in gastrointestinal stromal tumors: Kaifi, J. T., Strelow, A., et al.; Mod. Pathol. 19, 399 (2006), Abstract
- Mutation of TP53 gene is involved in carcinogenesis of hepatic undifferentiated (embryonal) sarcoma of the adult, in contrast with Wnt or telomerase pathways: an immunohistochemical study of three cases with genomic relation in two cases: Lepreux, S., Rebouissou, S., et al.; J. Hepatol. 42, 424 (2005), Abstract
- C-Reactive Protein in Atherosclerotic Lesions: Its Origin and Pathophysiological Significance: H. Sun, et al.; Am. J. Pathol. 167, 1139 (2005), Application(s): IHC on rabbit tissue sections, Abstract — Full Text
- Cytoplasmic expression and extracellular deposition of an antiangiogenic factor, pigment epithelium-derived factor, in human atherosclerotic plaques: Baba, H., Yonemitsu, Y., et al.; Arterioscler. Thromb. Vasc. Biol. 25, 1938 (2005), Abstract
- Regulation of macrophage foam cell formation by alphaVbeta3 integrin: potential role in human atherosclerosis: Antonov, A. S., Kolodgie, F. D., et al.; Am. J. Pathol. 165, 247 (2004), Abstract
- RGC-32 increases p34CDC2 kinase activity and entry of aortic smooth muscle cells into S-phase: Badea, T., Niculescu, F., et al.; J. Biol. Chem. 277, 502 (2002), Abstract
- Idiopathic hepatic fibrosis with cholestasis in broiler chickens: immunohistochemistry of hepatic stellate cells: Ochiai, K., Handharyani, E., et al.; Avian. Pathol. 31, 425 (2002), Abstract
- Basic fibroblast growth factor and its receptors in idiopathic pulmonary fibrosis and lymphangioleiomyomatosis: Inoue, Y., King, T. E., et al.; Am. J. Respir. Crit. Care Med. 166, 765 (2002), Abstract
- Nonneoplastic endometrial signet-ring cells. Vacuolated decidual cells and stromal histiocytes mimicking adenocarcinoma: Iezzoni, J. C., Mills, S. E., et al.; Am. J. Clin. Pathol. 115, 249 (2001), Abstract
- Sublytic C5b-9 induces proliferation of human aortic smooth muscle cells: Role of mitogen activated protein kinase and phosphatidylinositol 3-kinase: F. Niculescu, et al.; Atherosclerosis 142, 47 (1999), Application(s): IHC on primary human ASMC cultures , Abstract
- ACE inhibitor quinapril reduces the arterial expression of NF-kappaB-dependent proinflammatory factors but not of collagen I in a rabbit model of atherosclerosis: Hernández-Presa, M. A., Bustos, C., et al.; Am. J. Pathol. 153, 1825 (1998), Abstract
- Regulation of smooth muscle cell scavenger receptor expression in vivo by atherogenic diets and in vitro by cytokines: Li, H., Freeman, M. W., et al.; J. Clin. Invest. 95, 122 (1995), Abstract
- Overexpression of C-terminally but not N-terminally truncated Myb induces fibrosarcomas: a novel nonhematopoietic target cell for the myb oncogene: Press, R. D., Reddy, E. P., et al.; Mol. Cell. Biol. 14, 2278 (1994), Abstract
- Primary culture of smooth muscle cells from benign prostatic hyperplasia: Wu, Y. X., Kawabe, K., et al.; J. Smooth Muscle Res 30, 51 (1994), Abstract
- Induction of the autoantigen proliferating cell nuclear antigen in T lymphocytes by a mycobacterial antigen: Haftel, H. M., Chang, Y., et al.; J. Clin. Invest. 94, 1365 (1994), Abstract
- Myogenic regulatory protein expression in adult soft tissue sarcomas. A sensitive and specific marker of skeletal muscle differentiation: Tallini, G., Parham, D. M., et al.; Am. J. Pathol. 144, 693 (1994), Abstract
- Distinct patterns of expression of fibroblast growth factors and their receptors in human atheroma and nonatherosclerotic arteries. Association of acidic FGF with plaque microvessels and macrophages: Brogi, E., Winkles, J. A., et al.; J. Clin. Invest. 92, 2408 (1993), Abstract
- Expression of follistatin, an activin-binding protein, in vascular smooth muscle cells and arteriosclerotic lesions: Inoue, S., Orimo, A., et al.; Arterioscler. Thromb. 13, 1859 (1993), Abstract
- Thrombin receptor expression in normal and atherosclerotic human arteries: Nelken, N. A., Soifer, S. J., et al.; J. Clin. Invest. 90, 1614 (1992), Abstract
- Long-term expression of human adenosine deaminase in vascular smooth muscle cells of rats: a model for gene therapy: Lynch, C. M., Clowes, M. M., et al.; PNAS 89, 1138 (1992), Abstract
- Monocyte chemoattractant protein-1 in human atheromatous plaques: Nelken, N. A., Coughlin, S. R., et al.; J. Clin. Invest. 88, 1121 (1991), Abstract
- Chemically induced mammary gland adenomyoepitheliomas and myoepithelial carcinomas of mice. Immunohistochemical and ultrastructural features: S. Rehm; Am. J. Pathol. 136, 575 (1990), Abstract
- Monocyte migration into the subendothelial space of a coculture of adult human aortic endothelial and smooth muscle cells: Navab, M., Hough, G. P., et al.; J. Clin. Invest. 82, 1853 (1988), Abstract
- Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins: R.A. Schmidt, et al.; Am. J. Pathol. 131, 19 (1988), Abstract — Full Text
- Diagnosis of Rhabdomyosarcomas using HHF35, an anti-muscle actin monoclonal antibody: R.A. Schmidt, et al.; Lab. Invest. 56, 70A (1987)
- HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization: T. Tsukada, et al.; Am. J. Pathol. 126, 51 (1987), Abstract — Full Text
- HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues: T. Tsukada, et al.; Am. J. Pathol. 127, 389 (1987), Abstract — Full Text
- Human atherosclerosis. II. Immunocytochemical analysis of the cellular composition of human atherosclerotic lesions: A.M. Gown, et al.; Am. J. Pathol. 125, 191 (1986), Abstract — Full Text
- CGA-7 and HHF, two monoclonal antibodies that recognize muscle actin and react with adherent cells in human long-term bone marrow cultures: P. Charbord, et al.; Blood 66, 1138 (1985), Abstract — Full Text
Related Products
| Application | IHC (PS), WB |
|---|---|
| Host | Mouse |
| Isotype | IgG1 |
| Species Reactivity | Human, Monkey, Rabbit |
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