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MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit

ADI-950-100

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Superior multiplex IHC kit for efficient detection of multiple antigens in a single tissue sample.

Biotin-free nanopolymer-based system for superior multiplex antigen detection
Complete kit includes HIGHDEF^® Chromogens for sensitive, crisp 2-color development
Preserves precious tissue while reducing reagent and labor costs
Expandable with additional chromogens (sold separately)

The MULTIVIEW^® (mouse-HRP/rabbit-AP) IHC kit is a non-biotin one-step detection system suitable for demonstrating antigens in formalin-fixed paraffin-embedded tissues and cryostat sections. The kit may also be used with blood smears, cytosmears, and cell preparations.

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MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit Immunohistochemistry — Immunohistochemical analysis: Formalin fixed paraffin embedded human tonsil double stained using MULTIVIEW^® (mouse-HRP/rabbit-AP) IHC kit (Prod. no. ADI-950-100). Primary rabbit anti-Ki67 antibody was visualized with HIGHDEF^® red IHC chromogen (AP) (Prod. no. ADI-950-140), while primary mouse anti-HMW CK antibody was visualized with HIGHDEF^® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, sold separately), producing a distinct golden yellow color contrasting with the bright red.

Product Details

Application	IHC
Assay Time	~2 hours
Contents	Peroxidase Block, POLYVIEW^® IHC reagent (mouse-HRP), POLYVIEW^® IHC reagent (rabbit-AP), HIGHDEF^® IHC chromogen substrate (DAB, stable) buffer & chromogen, HIGHDEF^® red IHC chromogen (AP) buffer & chromogen, Empty mixing bottles
Technical Info / Product Notes	The MULTIVIEW^® (mouse-HRP/rabbit-AP) IHC kit has been developed by directly labeling anti-rabbit and anti-mouse immunoglobulins with enzymes using a proprietary tandem hyperlabelling technology. This ensures consistent and reproducible immunodetection of mouse and rabbit antibodies with a single reagent. Nuclear, cytoplasmic, and membrane antigens in different types of tissues can be readily detected. The MULTIVIEW^® (mouse-HRP/rabbit-AP) IHC kit enables faster staining procedures than traditional two-step methods using biotin and avidin/streptavidin conjugates by being able to directly label mouse antibodies with HRP and rabbit antibodies with AP at the same time, all with significantly lower background staining. The kit is suitable for use with mouse and rabbit antibodies, both monoclonal and polyclonal. The reagents can be used for manual staining or with automated staining instruments and is specifically designed for multiplex immunohistochemical staining assays.

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

X chromosome escapee genes are involved in ischemic sexual dimorphism through epigenetic modification of inflammatory signals: Qi, S., Al Mamun, A., et al.; J. Neuroinflammation 18, 70 (2021), Abstract
Activation of neuronal Ras-related C3 botulinum toxin substrate 1 (Rac1) improves post-stroke recovery and axonal plasticity in mice: Bu, F., Munshi, Y., et al.; J. Neurochem. 157, 1366 (2021), Abstract
AKAP12/Gravin is over-expressed in patients with ulcerative colitis: G. Fonseca-Camarillo, et al.; Immunol. Res. 69, 429 (2021), Abstract
Transglutaminase 2 promotes tumorigenicity of colon cancer cells by inactivation of the tumor suppressor p53: P. Malkomes, et al.; Oncogene 40, 4352 (2021), Abstract
Normalizing glucose levels reconfigures the mammary tumor immune and metabolic microenvironment and decreases metastatic seeding: H.A.M. Alsheikh, et al.; Cancer Lett. 517, 24 (2021), Abstract
Profiling of subcellular EGFR interactome reveals hnRNP A3 modulates nuclear EGFR localization: T.H. Wang, et al.; Oncogenesis 9, 40 (2020), Abstract — Full Text
Gelatinase B/Matrix Metalloproteinase-9 as Innate Immune Effector Molecule in Achalasia: J. Furuzawa-Carballeda, et al.; Clin. Transl. Gastroenterol. 9, 208 (2018), Application(s): Tissue biopsies from achalasia patients, Abstract — Full Text
MEF2‑activated long non‑coding RNA PCGEM1 promotes cell proliferation in hormone‑refractory prostate cancer through downregulation of miR‑148a: Zhang, S., Li, Z., et al.; Mol. Med. Rep. 18, 202 (2018), Abstract
Prevalence of polyreactive innate clones among graft-infiltrating B cells in human cardiac allograft vasculopathy: D. Chatterjee, et al.; J. Heart Lung Transplant. 37, 385 (2018), Application(s): Multiplex IHC staining on human cardiac tissue, Abstract
Caspase recruitment domain (CARD) family (CARD9, CARD10, CARD11, CARD14 and CARD15) are increased during active inflammation in patients with inflammatory bowel disease: J.K. Yamamoto-Furusho, et al.; J. Inflamm. (Lond.) 15, 13 (2018), Abstract — Full Text

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Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Specific Protocol

MULTIVIEW^® 4-color staining protocol available through our Technical Support webpages.

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MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit

ADI-950-100

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Specific Protocol

Certificate of Analysis

SDS

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MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit

ADI-950-100

Product Details

Handling & Storage

Related Products

HIGHDEF® blue IHC chromogen (AP)

ADI-950-150

HIGHDEF® Hematoxylin

ENZ-ACC106

POLYVIEW® PLUS AP (anti-rabbit) reagent

ENZ-ACC110

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Specific Protocol

Certificate of Analysis

SDS

HIGHDEF^® Hematoxylin

POLYVIEW^® PLUS AP (anti-rabbit) reagent