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mTOR (human FRB Domain) polyclonal antibody

ALX-215-065

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mTOR (FKBP12-rapamycin associated protein (FRAP)) is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. It has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. FKBP12-rapamycin-binding (FRB) domain is a conserved 11kDa region necessary for FKBP12-rapamycin binding. FRB is one of several functional domains of mTOR: HEAT (Huntington elongation factor 1A-protein phosphatase 2A-A subunit-TOR); FAT (FRAP, ATM, TTRAP2); PIKK (PI3-kinase-related kinase); RD (regulatory domain); and FATC (FAT, C terminal).

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20 & 25 µl loaded in lane 1 and 2 — **Figure:** WB using PAb to mTOR (human FRB Domain) (Prod. No. ALX-215-065) on total yeast extracts derived from cells expressing FRB-tagged genes coding for three different transcription factors. Separation SDS-PAGE.”””,mTOR (human FRB Domain) polyclonal antibody WB,mTOR (human FRB Domain) polyclonal antibody Western Blot,yes
ALX-215-065,215-065_2_WB.gif,http://static.enzolifesciences.com/fileadmin/files/image/215-065_2_WB.gif,**Figure 2:** Western Blot of mTOR (human FRB Domain)

mTOR (human FRB Domain) polyclonal antibody Western blot — **Figure 2:** Western Blot of mTOR (human FRB Domain), pAb (Prod. No. ALX-215-065) with Hela cells.
**Method:**Two ml of an exponential HeLa suspension culture (2-4×105 cells) was washed twice with PBS quickly without resuspention of the pellet by spinning in a Eppendorf tube and finally with 25% TCA. TCA was carefully removed and pellet respuspended 100µl FSB + ßME (final sample buffer) and the pH adjusted with about a few µl of 1M Tris base (≈10 µl). The sample was heated at 100°C for 5 min. and sonicated. SDS PAGE was at 7.5%, 20 & 25 µl loaded in lane 1 and 2, respectively. The TCA step is performed to prevent proteolytic breakdown of the very large mTOR.

Product Details

Alternative Name	mTOR (human FKBP12-rapamycin-binding domain)
Application	ChIP, ICC, WB
Application Notes	Among other applications this antibody can be used to control FRB-tagging of genes and the level of expression thereof in yeast cells (see H. Haruki, et al. 2008).
Formulation	Lyophilized from neat serum containing sodium azide.
Host	Rabbit
Immunogen	Recombinant human mTOR (FKBP12-rapamycin-binding (FRB) domain).
Recommendation Dilutions/Conditions	Western Blot (1:2,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Reconstitution	Reconstitute with 50µl water.
Species Reactivity	Human
Specificity	Recognizes the FRB domain of fusion proteins expressed in Saccharomyces cerevisiae (see H. Haruki, et al. 2008) and the human FRB domain of mTOR.
UniProt ID	P42345
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Anchor-Away: Efficient, Conditional Depletion of Nuclear Proteins in Saccharomyces cerevisiae: Xu, K., Côté, V., et al.; Methods Mol. Biol. 2919, 267 (2025), Abstract
Ubiquiton-An inducible, linkage-specific polyubiquitylation tool: Renz, C., Asimaki, E., et al.; Mol. Cell 84, 386 (2024), Abstract
The influence of flocculation upon global gene transcription in a yeast CYC8 mutant: Lee, B., Hokamp, K., et al.; Microb. Genom. 10, (2024), Abstract
A conserved function of corepressors is to nucleate assembly of the transcriptional preinitiation complex: Leydon, A. R., Downing, B., et al.; bioRxiv , (2024)
RNA polymerase II dynamics and mRNA stability feedback scale mRNA amounts with cell size: Swaffer, M. P., Marinov, G. K., et al.; Cell 186, 5254 (2023), Abstract
Engineered Extracellular Vesicles with Compound-Induced Cargo Delivery to Solid Tumors.: Kim, R., Kim, J. H., et al.; Int. J. Mol. Sci. 24, (2023), Application(s): WB, Abstract
RNA polymerase II dynamics and mRNA stability feedback scale mRNA in proportion to cell size: Swaffer, M. P., Marinov, G. K., et al.; bioRxiv , (2021)
Repression by the Arabidopsis TOPLESS corepressor requires association with the core mediator complex.: Zheng, N., Wang, W., et al.; Elife 10, (2021), Application(s): WB, Abstract
Active transcription and Orc1 drive chromatin association of the AAA+ ATPase Pch2 during meiotic G2/prophase.: Cardoso da Silva, R., Villar-Fernández, M. A., et al.; PLoS Genet. 16, e1008905 (2020), Application(s): WB, Abstract
Repression by the i>Arabidopsis/i> TOPLESS corepressor requires association with the core Mediator complex: Zheng, N., Wang, W., et al.; bioRxiv , (2020), Application(s): WB