mTOR (FKBP12-rapamycin associated protein (FRAP)) is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. It has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. FKBP12-rapamycin-binding (FRB) domain is a conserved 11kDa region necessary for FKBP12-rapamycin binding. FRB is one of several functional domains of mTOR: HEAT (Huntington elongation factor 1A-protein phosphatase 2A-A subunit-TOR); FAT (FRAP, ATM, TTRAP2); PIKK (PI3-kinase-related kinase); RD (regulatory domain); and FATC (FAT, C terminal).
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Figure: WB using PAb to mTOR (human FRB Domain) (Prod. No. ALX-215-065) on total yeast extracts derived from cells expressing FRB-tagged genes coding for three different transcription factors. Separation SDS-PAGE.”””,mTOR (human FRB Domain) polyclonal antibody WB,mTOR (human FRB Domain) polyclonal antibody Western Blot,yes
ALX-215-065,215-065_2_WB.gif,http://static.enzolifesciences.com/fileadmin/files/image/215-065_2_WB.gif,Figure 2: Western Blot of mTOR (human FRB Domain)

Figure 2: Western Blot of mTOR (human FRB Domain), pAb (Prod. No. ALX-215-065) with Hela cells.
Method:Two ml of an exponential HeLa suspension culture (2-4×105 cells) was washed twice with PBS quickly without resuspention of the pellet by spinning in a Eppendorf tube and finally with 25% TCA. TCA was carefully removed and pellet respuspended 100µl FSB + ßME (final sample buffer) and the pH adjusted with about a few µl of 1M Tris base (≈10 µl). The sample was heated at 100°C for 5 min. and sonicated. SDS PAGE was at 7.5%, 20 & 25 µl loaded in lane 1 and 2, respectively. The TCA step is performed to prevent proteolytic breakdown of the very large mTOR.


Product Details
Alternative Name |
mTOR (human FKBP12-rapamycin-binding domain) |
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Application |
ChIP, ICC, WB |
Application Notes |
Among other applications this antibody can be used to control FRB-tagging of genes and the level of expression thereof in yeast cells (see H. Haruki, et al. 2008). |
Formulation |
Lyophilized from neat serum containing sodium azide. |
Host |
Rabbit |
Immunogen |
Recombinant human mTOR (FKBP12-rapamycin-binding (FRB) domain). |
Recommendation Dilutions/Conditions |
Western Blot (1:2,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
Reconstitution |
Reconstitute with 50µl water. |
Species Reactivity |
Human |
Specificity |
Recognizes the FRB domain of fusion proteins expressed in Saccharomyces cerevisiae (see H. Haruki, et al. 2008) and the human FRB domain of mTOR. |
UniProt ID |
P42345 |
Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
Long Term Storage |
-20°C |
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Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- Anchor-Away: Efficient, Conditional Depletion of Nuclear Proteins in Saccharomyces cerevisiae: Xu, K., Côté, V., et al.; Methods Mol. Biol. 2919, 267 (2025), Abstract
- Ubiquiton-An inducible, linkage-specific polyubiquitylation tool: Renz, C., Asimaki, E., et al.; Mol. Cell 84, 386 (2024), Abstract
- The influence of flocculation upon global gene transcription in a yeast CYC8 mutant: Lee, B., Hokamp, K., et al.; Microb. Genom. 10, (2024), Abstract
- A conserved function of corepressors is to nucleate assembly of the transcriptional preinitiation complex: Leydon, A. R., Downing, B., et al.; bioRxiv , (2024)
- RNA polymerase II dynamics and mRNA stability feedback scale mRNA amounts with cell size: Swaffer, M. P., Marinov, G. K., et al.; Cell 186, 5254 (2023), Abstract
- Engineered Extracellular Vesicles with Compound-Induced Cargo Delivery to Solid Tumors.: Kim, R., Kim, J. H., et al.; Int. J. Mol. Sci. 24, (2023), Application(s): WB, Abstract
- RNA polymerase II dynamics and mRNA stability feedback scale mRNA in proportion to cell size: Swaffer, M. P., Marinov, G. K., et al.; bioRxiv , (2021)
- Repression by the Arabidopsis TOPLESS corepressor requires association with the core mediator complex.: Zheng, N., Wang, W., et al.; Elife 10, (2021), Application(s): WB, Abstract
- Active transcription and Orc1 drive chromatin association of the AAA+ ATPase Pch2 during meiotic G2/prophase.: Cardoso da Silva, R., Villar-Fernández, M. A., et al.; PLoS Genet. 16, e1008905 (2020), Application(s): WB, Abstract
- Repression by the i>Arabidopsis/i> TOPLESS corepressor requires association with the core Mediator complex: Zheng, N., Wang, W., et al.; bioRxiv , (2020), Application(s): WB
- Histone Recycling by FACT and Spt6 during Transcription Prevents the Scrambling of Histone Modifications.: Robert, F., Jeronimo, C., et al.; Cell Rep. 28, 1206 (2019), Application(s): WB, Abstract
- DiCre-Based inducible gene expression: J.D. Damasceno, et al.; Methods Mol. Biol. 1971, 225 (2019), Abstract
- Autoinhibitory regulation of S100A8/S100A9 alarminactivity locally restricts sterile inflammation: T. Vogl, et al.; J. Clin. Invest. 128, 1852 (2018), Application(s): Immunofluorescence on human alveolar epithelial cells, Abstract — Full Text
- Genome-wide Analysis of RNA Polymerase II Termination at Protein-Coding Genes: C. Baejen, et al.; Mol. Cell 66, 38 (2017), Abstract
- Evidence for Multiple Mediator Complexes in Yeast Independently Recruited by Activated Heat Shock Factor: J. Anandhakumar, et al.; Mol. Cell. Biol. 36, 1943 (2016), Application(s): ChIP from yeast cultures, Abstract — Full Text
- Kin28 regulates the transient association of Mediator with core promoters: C. Jeronimo & F. Robert; Nat. Struct. Mol. Biol. 21, 449 (2014), Application(s): ChIP from yeast cultures, Abstract — Full Text
- Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms: N. Andenmatten, et al.; Nat. Methods 10, 125 (2013), Application(s): WB in T. gondii samples, Abstract — Full Text
- Organelle-specific, rapid induction of molecular activities and membrane tethering: T. Komatsu, et al.; Nat. Methods 7, 206 (2010), Abstract — Full Text
- The anchor-away technique: rapid, conditional establishment of yeast mutant phenotypes: H. Haruki, et al.; Mol. Cell 31, 925 (2008), Abstract
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Last modified: January 23, 2025
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