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mtHSP70 monoclonal antibody (JG1)

ALX-804-077

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This antibody is covered by our Worry-Free Guarantee.

trans-Golgi network 38 monoclonal antibody (2F7.1) Immunofluorescence — **Figure:** Immunolocalization of mtHSP70 in human fibroblasts using MAb to to mtHSP70 (JG1) (Prod. No. ALX-804-077).”””,mtHSP70 monoclonal antibody (JG1) ICC,mtHSP70 monoclonal antibody (JG1) Immunocytochemistry,yes
ALX-804-095,804-095.gif,http://static.enzolifesciences.com/fileadmin/files/image/804-095.gif,**Figure:** Immunofluorescence staining of TGN38 (red) with MAb to trans-Golgi Network 38 (2F7.1) (Prod. No. ALX-804-095) and β-COP (green) with PAb to β-COP (Pro. No. ALX-210-127) in NRK cells.”””””

Product Details

Alternative Name	Mortalin, Heat shock protein 70 (mitochondrial), GRP75
Application	ICC, IP, WB
Application Notes	Detects a band of ~75kDa by Western blot.
Clone	JG1
Formulation	Liquid. In PBS with 1 mg/mL BSA containing 0.05% sodium azide.
Host	Mouse
Immunogen	Synthetic peptide corresponding to C-terminal aa 615-633 (G615SGSSGTGEQKEDQKEEKQ633) of mouse mtHSP70 (mitochondrial heat shock protein 70).
Isotype	IgG3
Purity Detail	Protein A purified.
Recommendation Dilutions/Conditions	Immunocytochemistry (1:50)Western Blot (1:500)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Dog, Human, Monkey, Mouse
UniProt ID	P38647
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

In-depth study of MPV17: a molecular travel unveiling a mitochondrial calcium regulation function: Meurant, S., Amato, I., et al.; bioRxiv , (2024), Application(s): ICC-IF / Reactant(s): Human
Endogenous TOM20 Proximity Labeling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human Cells.: Meurant, S., Mauclet, L., et al.; Int. J. Mol. Sci. 24, (2023), Application(s): ICC-IF, Abstract
Dietary apple polyphenols enhance mitochondrial turnover and respiratory chain enzymes: Yoshida, Y., Tamura, Y., et al.; Exp. Physiol. 108, 1295 (2023), Abstract
Endogenous TOM20 Proximity Labelling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human Cells: Meurant, S., Mauclet, L., et al.; Preprints.org , (2023), Application(s): ICC-IF
Neuralized-like protein 4 (NEURL4) mediates ADP-ribosylation of mitochondrial proteins: M.D. Cardamone, et al.; J. Cell Biol. 221, e202101021 (2022), Application(s): WB, Abstract
Ozone Activates the Nrf2 Pathway and Improves Preservation of Explanted Adipose Tissue In Vitro: B. Cisterna, et al.; Antioxidants (Basel) 9, 989 (2020), Application(s): WB / Reactant(s) Mouse, Abstract — Full Text
Effects of Heat Stress Treatment on Age-dependent Unfolded Protein Response in Different Types of Skeletal Muscle.: Hatta, H., Tamura, Y., et al.; J. Gerontol. A Biol. Sci. Med. Sci. 72, 299 (2017), Application(s): WB / Reactant(s): Mouse, Abstract
Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population: Vannuvel, K., Van Steenbrugge, M., et al.; J. Cell. Physiol. 231, 1913 (2016), Abstract
HDAC6 maintains mitochondrial connectivity under hypoxic stress by suppressing MARCH5/MITOL dependent MFN2 degradation: H.J. Kim, et al.; Biochem. Biophys. Res. Commun. 464, 1235 (2015), Application(s): Immunostaining, Abstract
Characterization of stress response in human retinal epithelial cells: V. Giansanti, et al.; J. Cell. Mol. Med. 17, 103 (2013), Application(s): ICC on human adult retinal pigmented epithelial (ARPE-19) cells, Abstract — Full Text

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mtHSP70 monoclonal antibody (JG1)

ALX-804-077

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