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Expression of selected transporters and tight junction proteins in brain capillaries of mice exposed to bilateral renal ischemia/reperfusion injury at the protein level. Brain capillaries were isolated from frozen brains of mice exposed to bilateral renal ischemia/reperfusion injury followed by protein extraction and Western blot (A). The protein content of Bcrp (Abcg2, ATP Binding Cassette Subfamily G Member 2) (B), Glut1 (Slc2a1, Solute Carrier Family 2 Member 1) (C), Mrp4 (Abcc4, ATP Binding Cassette Subfamily C Member 4) (D), and P-gp (Mdr1, Abcb1b ATP Binding Cassette Subfamily B Member1) (E) was normalized to β-actin and is shown as average of n = 3 with standard deviations. *p < 0.05, ***p < 0.001.
Image collected and cropped by CiteAb under a CC-BY license from the following publication: Kidney Ischemia/Reperfusion Injury Induces Changes in the Drug Transporter Expression at the Blood-Brain Barrier in vivo and in vitro. Front Physiol (2020)
Effects of ischemic HK-2 and uremic toxins on efflux pumps and cellular receptor protein level in brain microvascular endothelial cells. BMECs treated as described in Figure 1 were lysed and used for protein extraction. The protein content of Mrp4 (Abcc4, ATP Binding Cassette Subfamily C Member 4), Tfrc (Transferrin Receptor), and Lrp1 (LDL Receptor Related Protein 1) was measured by Western blot (A). The protein content was normalized to β-actin and to the untreated control. The densitometric analysis is shown as an average of four independent experiments with standard deviations (B–D). One-way ANOVA with Dunnett’s or Sidak’s multiple comparison test was used to compare each treatment to the untreated control. *p < 0.05.
Image collected and cropped by CiteAb under a CC-BY license from the following publication: Kidney Ischemia/Reperfusion Injury Induces Changes in the Drug Transporter Expression at the Blood-Brain Barrier in vivo and in vitro. Front Physiol (2020)
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RUO – Research Use Only |
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