Monobromobimane (ultra pure)

Monobromobimane (mBBr) is a thiol-reactive fluorescent probe used for the determination of the redox status of low molecular weight and protein thiols in biological systems. Monobromobimane-based in situ derivatization results in maximal recovery of both free, reduced low molecular weight and monobromobimane-accessible protein thiols. The quantitation of the corresponding adducts of protein thiols is achieved by fluorescence spectroscopy, following protein precipitation. The reagent is particularly useful for quantifying glutathione. Wavelength Maxima: Excitation 395nm, Emission 490nm