Home Shop Mono- and polyubiquitinylated conjugates recombinant monoclonal antibody (UBCJ2)

Mono- and polyubiquitinylated conjugates recombinant monoclonal antibody (UBCJ2)

ENZ-ABS840

36 citations

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This antibody is covered by our Worry-Free Guarantee.

Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (HRP conjugate) Western Blot 2 — Western Blot analysis of ENZ-ABS840HRP detecting ubiquitin chains: Lane 1: MW Marker, Lane 2: K48-linked, Lane 3: K63-linked.

Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (FITC conjugate) Immunocytochemistry — Immunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using FITC labeled mono- and polyubiquitinylated conjugates mAb (UBCJ2), (Prod. No. ENZ-ABS840F): HeLa cells, pre-treated with proteasome inhibitor MG-132 (Prod. No. BML-PI102, or ENZ-51035) at 5μM for 12 hours prior to analysis with antibody at 1:250 dilution. Panel A: isotype control (Prod. No. ADI-SAB-600FI); Panel B: untreated; Panel C: proteasome inhibitor-treated HeLa cells. Left: 20X and Right: 63X amplified.

Mono- and polyubiquitinylated conjugates recombinant monoclonal antibody (UBCJ2) Western Blot — Western Blot analysis of ubiquitinylated conjugates in whole cell lysates using the Mono- and polyubiquitinylated conjugates mAb (UBCJ2) (Prod. No. ENZ-ABS840). HeLa cells were treated for 12 hours with DMSO (vehicle, lane 1) or proteasome inhibitor MG-132 at 25 µM (lane 2). Membrane was incubated O.N. (4°C) with 1 µg/mL of mAb clone UBCJ2. Detection was done with a goat anti-mouse secondary antibody. Ponceau-S staining was performed as loading control (bottom panel). Image is a courtesy of Dr Jacob Seeler, Nuclear Organization and Oncogenesis Unit, Pasteur Institute, Paris (FR).

Mono- and polyubiquitinylated conjugates monoclonal antibody (UBCJ2) (FITC conjugate) Flow Cytometry — Flow cytometry analysis of 106 Jurkat cells stained using Mono- and polyubiquitinylated conjugates mAb (UBCJ2) (FITC conjugate), (Prod. No. ENZ-ABS840F), at a concentration of 10µg/ml, side-by-side comparison to Mono- and polyubiquitinylated conjugates mAb (FK2) (fluorescein labeled), (Prod. No. BML-PW1210).

Product Details

Application	ELISA, Flow Cytometry, ICC, WB
Clone	UBCJ2
Formulation	Liquid. In PBS containing 0.09% sodium azide.
Host	Mouse
Immunogen	Poly-ubiquitinylated lysozyme.
Isotype	IgG1κ
Purity Detail	Protein A affinity purified.
Recommendation Dilutions/Conditions	ELISA (1:1000)Immunocytochemistry (1:250)Flow cytometry (1:100)Western Blot (1:1000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from tissue culture supernatant.
Species Reactivity	Species independent
Specificity	Recognizes mono- and polyubiquitinylated protein conjugates in a wide range of species.
UniProt ID	P0CG47 (UBB), P0CG48 (UBC)
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Store unopened vial at -20°C. Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Nutrient status alters developmental fates via a switch in mitochondrial homeodynamics.: Zhang, J., Liu, S. N., et al.; Nat. Commun. 16, 1258 (2025), Application(s): IHC / Reactant(s): Drosophila melanogaster, Abstract
Influenza A virus subverts the LC3-pericentrin dynein adaptor complex for host cytoplasm entry: Cong, Y., Verlhac, P., et al.; Sci. Adv. 11, eadu7602 (2025), Abstract
p97/VCP is required for piecemeal autophagy of aggresomes: Körner, M., Müller, P., et al.; Nat. Commun. 16, 4243 (2025), Reactant(s): Human, Abstract
FOXO-regulated DEAF1 controls muscle regeneration through autophagy: Goh, K. Y., Lee, W. X., et al.; Autophagy 20, 2632 (2024), Abstract
FOXO-regulated Deaf1 controls muscle regeneration through autophagy: Goh, K. Y., Lee, W. X., et al.; bioRxiv , (2024)
Host E3 ubiquitin ligase ITCH mediates Toxoplasma gondii effector GRA35-triggered NLRP1 inflammasome activation and cell-autonomous immunity: Wang, Y., Hollingsworth, L. R., et al.; Mbio 15, e0330223 (2024), Abstract
Mitochondrial outer membrane integrity regulates a ubiquitin-dependent and NF-κB-mediated inflammatory response: Vringer, E., Heilig, R., et al.; EMBO J. 43, 904 (2024), Abstract
Mitochondrial outer membrane integrity regulates a ubiquitin-dependent NF-κB inflammatory response: Vringer, E., Riley, J. S., et al.; bioRxiv , (2023)
PINK1, Keap1, and Rtnl1 regulate selective clearance of endoplasmic reticulum during development: Wang, R., Fortier, T. M., et al.; Cell 186, 4172 (2023), Abstract
Caveolin-1 dolines form a distinct and rapid caveolae-independent mechanoadaptation system: Lolo, F. N., Walani, N., et al.; Nat. Cell Biol. 25, 120 (2023), Abstract

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Last modified: May 29, 2024

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Mono- and polyubiquitinylated conjugates recombinant monoclonal antibody (UBCJ2)

ENZ-ABS840

Product Details

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